Capillary isoelectric focusing without carrier ampholytes

被引：40

作者：

Huang, TM ^{[1
]}

Wu, XZ ^{[1
]}

Pawliszyn, J ^{[1
]}

机构：

[1] Univ Waterloo, Guelph Waterloo Ctr Grad Work Chem, Dept Chem, Waterloo, ON N2L 3G1, Canada

来源：

ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 19期

关键词：

D O I：

10.1021/ac000599l

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel capillary isoelectric focusing (CIEF) separation of proteins in pure water was investigated using the whole-column imaging detection technique. Protons and hydroxyl ions are produced by the electrolysis of water, and the pH gradient is created by the electromigration of protons and hydroxyl ions into the separation capillary. The addition of acids to the anodic solution and bases to the cathodic solution enhances the formation of the gradients. Fast CIEF separation of proteins by electrolysis of water is demonstrated.

引用

页码：4758 / 4761

页数：4

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