Diurnal variations in the activities of hydrolytic enzymes in different fractions of rumen contents of Murrah buffalo

被引:72
作者
Agarwal, N [1 ]
Agarwal, I [1 ]
Kamra, DN [1 ]
Chaudhary, LC [1 ]
机构
[1] Indian Vet Res Inst, Ctr Adv Studies Anim Nutr, Microbiol Sect, Izatnagar 243122, Uttar Pradesh, India
关键词
Buffalo; rumen contents; carboxymethylcellulase; alpha-amylase; xylanase; microcrystalline cellulase; filter paper degrading activity; rumen enzymes;
D O I
10.1080/09712119.2000.9706325
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
The hydrolytic enzymes like carboxymethylcellulase, alpha-amylase, xylanase, microcrystalline cellulase and filter paper degrading activities were estimated in various fractions of rumen contents of Murrah buffalo. About 80-92% of the enzyme activities were associated with the particulate material, while only a negligible portion. (1-4%) was present as extracellular fraction in the liquid portion of rumen contents. Rest of the activities (8-15%) were associated with freely suspended microbial cells irt the liquid portion of the rumen contents. Maximum activities of carboxymethylcellulase, alpha-amylase, xylanase and micro-crystalline cellulase were observed at 2h post feeding while in the case of filter paper degrading activity, there was no difference in the activities in the first 4 hours of offering feed to the animals.
引用
收藏
页码:73 / 80
页数:8
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