Seasonal cell proliferation in the chemosensory epithelium and brain of red-backed salamanders, Plethodon cinereus

被引:30
作者
Dawley, EM [1 ]
Fingerlin, A [1 ]
Hwang, D [1 ]
John, SS [1 ]
Stankiewicz, CA [1 ]
机构
[1] Ursinus Coll, Dept Biol, Collegeville, PA 19426 USA
关键词
cell proliferation; chemosensory epithelium; olfactory; vomeronasal; periventricular; salamanders; seasonal;
D O I
10.1159/000006673
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
The chemosensory epithelium of vertebrates retains the ability to produce new receptor neurons throughout life, presumably as a mechanism to replace aging or damaged receptors. We examined cell division in the main olfactory and vomeronasal epithelia of red-backed salamanders (Plethodon cinereus) because previous studies had shown that the volume of sensory epithelia changes seasonally. Cell division was compared throughout the year by injecting salamanders once with 5-bromo-2 '-deoxyuridine (BrdU), which is incorporated into the DNA of cells during DNA synthesis, and sacrificing them one hour after injection. We used immunocytochemistry to locate cells that had arisen from cell division since BrdU injection and compared the number of labeled cells per area among animals. Animals collected in May had significantly more labeled nuclei than animals collected in any other month. However, proliferation rates among the other months were not significantly different and were quite low. Labeled nuclei also were found around the cerebral ventricles of salamanders collected in May, but rarely in any other month, although other tissues in the head often were heavily labeled. Cell proliferation appears to be up-regulated in the chemosensory epithelia and in the telencephalon during May, and we hypothesize that new receptors, and perhaps their interneurons in the telencephalon, are being generated in anticipation of seasonal events that are mediated by chemoreception. Copyright (C) 2000 S. Karger AG, Basel.
引用
收藏
页码:1 / 13
页数:13
相关论文
共 67 条
[1]  
[Anonymous], 2007, Biostatistical analysis
[2]  
Arnold S.J., 1977, P141
[3]   PREMITOTIC DNA-SYNTHESIS IN THE BRAIN OF THE ADULT FROG (RANA-ESCULENTA L) - AN AUTORADIOGRAPHIC 3H-THYMIDINE STUDY [J].
BERNOCCHI, G ;
SCHERINI, E ;
GIACOMETTI, S ;
MARES, V .
ANATOMICAL RECORD, 1990, 228 (04) :461-470
[4]  
BOWERS SM, 1988, THESIS YALE U NEW HA
[5]  
BURTON TM, 1975, COPEIA, P541
[6]  
Calof AL, 1996, J NEUROBIOL, V30, P67
[7]   ABLATION OF THE OLFACTORY-BULB UP-REGULATES THE RATE OF NEUROGENESIS AND INDUCES PRECOCIOUS CELL-DEATH IN OLFACTORY EPITHELIUM [J].
CARR, VM ;
FARBMAN, AI .
EXPERIMENTAL NEUROLOGY, 1992, 115 (01) :55-59
[8]   ULTRASTRUCTURAL AUTORADIOGRAPHIC ANALYSIS OF NEUROGENESIS IN THE HYPOTHALAMUS OF THE ADULT FROG, RANA-TEMPORARIA, WITH SPECIAL REFERENCE TO PHYSIOLOGICAL REGENERATION OF THE PREOPTIC NUCLEUS .1. VENTRICULAR ZONE CELL-PROLIFERATION [J].
CHETVERUKHIN, VK ;
POLENOV, AL .
CELL AND TISSUE RESEARCH, 1993, 271 (02) :341-350
[9]   SEXUAL AND SEASONAL DIFFERENCES IN THE VOMERONASAL EPITHELIUM OF THE RED-BACKED SALAMANDER (PLETHODON-CINEREUS) [J].
DAWLEY, EM ;
CROWDER, J .
JOURNAL OF COMPARATIVE NEUROLOGY, 1995, 359 (03) :382-390
[10]   ORGANIZATION OF THE VOMERONASAL ORGAN IN A PLETHODONTID SALAMANDER [J].
DAWLEY, EM ;
BASS, AH .
JOURNAL OF MORPHOLOGY, 1988, 198 (02) :243-255