Application of the standard addition approach for the quantification of urinary benzene

被引:41
作者
Basilicata, P
Miraglia, N
Pieri, M
Acampora, A
Soleo, L
Sannolo, N
机构
[1] Univ Naples Federico II, Dipartimento Med Pubbl & Sicurezza Sociale, I-80131 Naples, Italy
[2] Univ Naples 2, Dipartimento Med Sperimentale, Sez Med Lavoro, I-80138 Naples, Italy
[3] Univ Bari, Dipartimento Med Interna & Med Pubbl, I-70126 Bari, Italy
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2005年 / 818卷 / 02期
关键词
urinary benzene; standard addition approach; occupational exposure;
D O I
10.1016/j.jchromb.2005.01.013
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Urinary benzene is used as biomarker of exposure to evaluate the uptake of this solvent both in non-occupationally exposed population and in benzene-exposed workers. The quantitative determination of benzene in urine is carried out in a three steps procedure: urine collection, sample analysis by head space/solid phase microextraction/gas chromatography/mass spectrometry and analyte quantification. The adopted quantification method influences the initial step, hence the whole procedure. Two quantification approaches were compared as regards precision and accuracy: the calibration curves and the standard addition method. Even if calibration curves obtained by using urine samples from different subjects were always linear, their slopes and intercepts showed noteworthy variations, attributable to the influence of the biological matrix on benzene recovery. The standard addition method showed to be more suitable for compensating matrix effects, and a three-point standard addition protocol was used to quantify benzene in urine samples of 11 benzene-exposed workers (smokers and nonsmokers). Urine from occupationally exposed workers was collected before and after work-shift. Besides urinary benzene, the applicability of the method was verified by measuring the urinary concentration of the S-phenylmercapturic acid, a specific benzene metabolite, generally adopted as biomarker in biological monitoring procedures. A similar trend of concentration levels of both analytes measured in urine samples collected before work-shift with respect to the after work-shift ones was found, showing the actual applicability of the standard addition method for biological monitoring purposes. (c) 2005 Elsevier B.V. All fights reserved.
引用
收藏
页码:293 / 299
页数:7
相关论文
共 26 条
[1]   HEMATOTOXICITY AND CARCINOGENICITY OF BENZENE [J].
AKSOY, M .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1989, 82 :193-197
[2]   Quantitative analysis of benzene, toluene, and xylenes in urine by means of headspace solid-phase microextraction [J].
Alkalde, TK ;
Peralba, MDCR ;
Zini, CA ;
Caramao, EB .
JOURNAL OF CHROMATOGRAPHY A, 2004, 1027 (1-2) :37-40
[3]  
American Conference of Governmental Industrial Hygienists,, 2001, AM C GOV IND HYG CIN, Vseventh
[4]  
[Anonymous], 1986, ENV POL SUS DEV
[6]  
CRUMP KS, 1992, J TOXICOL ENV HLTH, V42, P219
[7]   Benzene-induced chromosome aberrations: A follow-up study [J].
Forni, A .
ENVIRONMENTAL HEALTH PERSPECTIVES, 1996, 104 :1309-1312
[8]   Headspace solid-phase microextraction for the determination of benzene, toluene, ethylbenzene and xylenes in urine [J].
Fustinoni, S ;
Giampiccolo, R ;
Pulvirenti, S ;
Buratti, M ;
Colombi, A .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1999, 723 (1-2) :105-115
[9]   EFFECT OF BENZENE IN THE MICRONUCLEUS TEST [J].
HITE, M ;
PECHARO, M ;
SMITH, I ;
THORNTON, S .
MUTATION RESEARCH, 1980, 77 (02) :149-155
[10]  
*ICPS, 1993, ENV HLTH CRIT, V150, P41