Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

被引：114

作者：

van de Pol, Alma C.

van Loon, Anton M.

Wolfs, Tom F. W.

Jansen, Nicolaas J. G.

Nijhuis, Monique

Breteler, Els Klein

Schuurman, Rob

Rossen, John W. A.

机构：

[1] St Elizabeth Hosp, Lab Med Microbiol & Immunol, NL-5022 GC Tilburg, Netherlands

[2] Univ Med Ctr Utrecht, Eijkman Winkler Ctr, Dept Virol, NL-3584 CX Utrecht, Netherlands

[3] Univ Med Ctr Utrecht, Wilhelmina Childrens Hosp, Div Infect Dis, Dept Pediat, NL-3584 EA Utrecht, Netherlands

[4] Univ Med Ctr Utrecht, Wilhelmina Childrens Hosp, Div Intens Care, Dept Pediat, NL-3584 EA Utrecht, Netherlands

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 07期

关键词：

D O I：

10.1128/JCM.00848-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

引用

收藏

页码：2260 / 2262

页数：3

相关论文

共 10 条

[1] Prospective study of respiratory viral infections in pediatric hemopoietic stem cell transplantation patients [J].

Bredius, RGM ;

Templeton, KE ;

Scheltinga, SA ;

Claas, ECJ ;

Kroes, ACM ;

Vossen, JM .

PEDIATRIC INFECTIOUS DISEASE JOURNAL, 2004, 23 (06) :518-522

[2] Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults [J].

Casiano-Colón, AE ;

Hulbert, BB ;

Mayer, TK ;

Walsh, EE ;

Falsey, AR .

JOURNAL OF CLINICAL VIROLOGY, 2003, 28 (02) :169-174

[3]

McAdam AJ, 2000, J CLIN MICROBIOL, V38, P2027

[4] Molecular genetic methods in the diagnosis of lower respiratory tract infections [J].

Murdoch, DR .

APMIS, 2004, 112 (11-12) :713-727

[5] Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza A and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4 [J].

Templeton, KE ;

Scheltinga, SA ;

Beersma, MFC ;

Kroes, ACM ;

Claas, ECJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2004, 42 (04) :1564-1569

[6] Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection [J].

van de Pol, Alma C. ;

Wolfs, Tom F. W. ;

Jansen, Nicolaas J. G. ;

van Loon, Anton M. ;

Rossen, John W. A. .

CRITICAL CARE, 2006, 10 (02)

[7] Applicability of a real-time quantitative PCR assay for diagnosis of respiratory syncytial virus infection in immunocompromised adults [J].

van Elden, LJR ;

van Loon, AM ;

van der Beek, A ;

Hendriksen, KAW ;

Hoepelman, AIM ;

van Kraaij, MGJ ;

Schipper, P ;

Nijhuis, M .

JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (09) :4378-4381

[8] Simultaneous detection of influenza viruses A and B using real-time quantitative PCR [J].

van Elden, LJR ;

Nijhuis, M ;

Schipper, P ;

Schuurman, R ;

van Loon, AM .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :196-200

[9] Frequent detection of respiratory viruses in adult recipients of stem cell transplants with the use of real-time polymerase chain reaction, compared with viral culture [J].

van Kraaij, MGJ ;

van Elden, LJR ;

van Loon, AM ;

Hendriksen, KAW ;

Laterveer, L ;

Dekker, AW ;

Nijhuis, M .

CLINICAL INFECTIOUS DISEASES, 2005, 40 (05) :662-669

[10] Design and performance testing of quantitative real time PCR assays for influenza A and B viral load measurement [J].

Ward, CL ;

Dempsey, MH ;

Ring, CJA ;

Kempson, RE ;

Zhang, L ;

Gor, D ;

Snowden, BW ;

Tisdale, M .

JOURNAL OF CLINICAL VIROLOGY, 2004, 29 (03) :179-188