Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms

被引:114
作者
van de Pol, Alma C.
van Loon, Anton M.
Wolfs, Tom F. W.
Jansen, Nicolaas J. G.
Nijhuis, Monique
Breteler, Els Klein
Schuurman, Rob
Rossen, John W. A.
机构
[1] St Elizabeth Hosp, Lab Med Microbiol & Immunol, NL-5022 GC Tilburg, Netherlands
[2] Univ Med Ctr Utrecht, Eijkman Winkler Ctr, Dept Virol, NL-3584 CX Utrecht, Netherlands
[3] Univ Med Ctr Utrecht, Wilhelmina Childrens Hosp, Div Infect Dis, Dept Pediat, NL-3584 EA Utrecht, Netherlands
[4] Univ Med Ctr Utrecht, Wilhelmina Childrens Hosp, Div Intens Care, Dept Pediat, NL-3584 EA Utrecht, Netherlands
关键词
D O I
10.1128/JCM.00848-07
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.
引用
收藏
页码:2260 / 2262
页数:3
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