Enrichment and biofilm formation of Anammox bacteria in a non-woven membrane reactor

被引：78

作者：

Ni, Shou-Qing ^{[2
]}

Lee, Po-Heng ^{[1
]}

Fessehaie, Anania ^{[3
]}

Gao, Bao-Yu ^{[2
]}

Sung, Shihwu ^{[1
]}

机构：

[1] Iowa State Univ, Dept Civil Construct & Environm Engn, Ames, IA 50011 USA

[2] Shandong Univ, Sch Environm Sci & Engn, Jinan 250100, Shandong, Peoples R China

[3] Iowa State Univ, Seed Sci Ctr, Ames, IA 50011 USA

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 06期

关键词：

Anammox; Biofilm; Enrichment; Nitrogen removal; Non-woven membrane reactor; AUTOTROPHIC NITROGEN-REMOVAL; AMMONIUM OXIDATION ANAMMOX; BLANKET UASB REACTORS; GRANULAR SLUDGE; ACTIVATED-SLUDGE; GAS-PRODUCTION; WASTE-WATER; CANON; MICROORGANISMS; TEMPERATURE;

D O I：

10.1016/j.biortech.2009.10.050

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

An innovative reactor configuration for Anammox enrichment by connecting a non-woven membrane module with an anaerobic reactor was developed in this study. The Anammox non-woven membrane reactor (ANMR) exhibited high biomass retention ability through the formation of aggregates in the reactor and biofilm on the interior surface of the non-woven membrane. No fouling problems occurred on the membrane after the development of mature biofilms. After 8 months of operation, the nitrogen loading rate (NLR) and nitrogen removal rate (NRR) reached 1263 mg N/l/d and 1047.5 mg N/l/d, respectively, with a maximum specific ammonium consumption (SAC) of 51 nmol/mg protein/min. At steady state, the average ammonium and nitrite removal efficiencies were 90.9% and 95.0%, respectively. Morphological observation of Anammox aggregates and biofilm showed a high degree of compactness. Also, enrichment of Anammox bacteria was quantified by real-time polymerase chain reaction (PCR) analysis as 97.7%. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：1792 / 1799

页数：8

共 37 条

[1]

Abma W., 2005, P 3 IWA LEAD EDG C E

[2] Full-scale granular sludge Anammox process [J].

Abma, W. R. ;

Schultz, C. E. ;

Mulder, J. W. ;

van der Star, W. R. L. ;

Strous, M. ;

Tokutomi, T. ;

van Loosdrecht, M. C. M. .

WATER SCIENCE AND TECHNOLOGY, 2007, 55 (8-9) :27-33

[3] A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide [J].

Allen, G. C. ;

Flores-Vergara, M. A. ;

Krasnyanski, S. ;

Kumar, S. ;

Thompson, W. F. .

NATURE PROTOCOLS, 2006, 1 (05) :2320-2325

[4]

[Anonymous], STAND METH EX WAT WA

[5] Structural analysis of anaerobic granules in a phase separated reactor by electron microscopy [J].

Baloch, M. I. ;

Akunna, J. C. ;

Kierans, M. ;

Collier, P. J. .

BIORESOURCE TECHNOLOGY, 2008, 99 (05) :922-929

[6] Inactivation of ANAMMOX communities under concurrent operation of anaerobic ammonium oxidation (ANAMMOX) and denitrification [J].

Chamchoi, N. ;

Nitisoravut, S. ;

Schmidt, J. E. .

BIORESOURCE TECHNOLOGY, 2008, 99 (09) :3331-3336

[7] Anammox enrichment from different conventional sludges [J].

Chamchoi, Nutchanat ;

Nitisoravut, Suwanchai .

CHEMOSPHERE, 2007, 66 (11) :2225-2232

[8] Low-cost membranes for use in a submerged MBR [J].

Chang, IS ;

Gander, M ;

Jefferson, B ;

Judd, SJ .

PROCESS SAFETY AND ENVIRONMENTAL PROTECTION, 2001, 79 (B3) :183-188

[9] Evaluation of activity and inhibition effects on Anammox process by batch tests based on the nitrogen gas production [J].

Dapena-Mora, A. ;

Fernandez, I. ;

Campos, J. L. ;

Mosquera-Corral, A. ;

Mendez, R. ;

Jetten, M. S. M. .

ENZYME AND MICROBIAL TECHNOLOGY, 2007, 40 (04) :859-865

[10] Enrichment of Anammox biomass from municipal activated sludge:: experimental and modelling results [J].

Dapena-Mora, A ;

Van Hulle, SWH ;

Campos, JL ;

Méndez, R ;

Vanrolleghem, PA ;

Jetten, M .

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY, 2004, 79 (12) :1421-1428

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