Comparison of QIAGEN Automated Nucleic Acid Extraction Methods for CMV Quantitative PCR Testing

被引：24

作者：

Miller, Steve ^{[1
]}

Seet, Henrietta ^{[1
]}

Khan, Yasmeen ^{[1
]}

Wright, Carolyn ^{[1
]}

Nadarajah, Rohan ^{[1
]}

机构：

[1] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2010年 / 133卷 / 04期

关键词：

Cytomegalovirus; Real-time polymerase chain reaction; Nucleic acid extraction; Viremia; Transplana monitoring; REAL-TIME PCR; CYTOMEGALOVIRUS-INFECTION; VIRAL LOAD; PP65; ANTIGENEMIA; TRANSPLANTATION; PLASMA; ASSAY; QUANTIFICATION; THERAPY; DNA;

D O I：

10.1309/AJCPE5VZL1ONZHFJ

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

We examined the effect of nucleic acid extraction methods on the analytic characteristics of a quantitative polymerase chain reaction (PCR) assay for cytomegalovirus (CMV) Human serum samples were extracted with 2 automated instruments (BioRobot EZI and QIAsymphony SP, QIAGEN, Valencia, CA) and CMV PCR results compared with those of pp65 amtogenemia testing Both extraction methods yielded results that were comparably linear and precise, wherease the QIAsymphony SP had a slightly lower limit of detection (1 92 log(10) copies/mL vs 2 26 log(10) copies/mL) In both cases, PCR was more sensitive than CMV antigen detection, detecting CMV viremia in 12% (EZI) and 21% (QIAsymphony) of antigen-negative specimens This study demonstrates the feasibility of using 2 different extraction techniques to yield results within 0 5 log(10) copies/mL of the mean value, a level that would allow for clinical comparison between different laboratory assays

引用

页码：558 / 563

页数：6

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