Role of vascular endothelial growth factor in bone marrow stromal cell modulation of endothelial cells

被引:152
作者
Kaigler, D
Krebsbach, PH
Polverini, PJ
Mooney, DJ
机构
[1] Univ Michigan, Dept Biol & Mat Sci, Ann Arbor, MI 48109 USA
[2] Univ Michigan, Dept Oral Med Pathol Oncol, Ann Arbor, MI 48109 USA
[3] Univ Minnesota, Sch Dent, Minneapolis, MN 55455 USA
[4] Univ Michigan, Dept Chem & Biomed Engn, Ann Arbor, MI 48109 USA
来源
TISSUE ENGINEERING | 2003年 / 9卷 / 01期
关键词
D O I
10.1089/107632703762687573
中图分类号
Q813 [细胞工程];
学科分类号
摘要
One of the fundamental principles that underlies tissue-engineering strategies using cell transplantation is that a newly formed tissue must acquire and maintain sufficient vascularization in order to support its growth. Enhancing angiogenesis through delivery of growth factors is one approach to establishing a vascular network to these tissues. In this study, we tested the potential of bone marrow stromal cells (BMSCs) to modulate the growth and differentiation activities of blood vessel precursors, endothelial cells (ECs), by their secretion of soluble angiogenic factors. The growth and differentiation of cultured ECs were enhanced in response to exposure to BMSC conditioned medium (CM). Enzyme-linked immunosorbent assays demonstrated that both mouse and human BMSCs secreted significant quantities of vascular endothelial growth factor (VEGF) (2.4-3.1 ng/10(6) cells per day). Furthermore, eliminating the activity of BMSC-secreted VEGF with blocking antibodies completely blocked the CM effects on cultured ECs. These data demonstrate that human BMSCs secrete sufficient quantities of VEGF to enhance survival and differentiation of endothelial cells in vitro, and suggest they may be capable of directly orchestrating angiogenesis in vivo.
引用
收藏
页码:95 / 103
页数:9
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