Effect of indomethacin on cell cycle dependent cyclic AMP fluxes in tobacco BY-2 cells

被引：70

作者：

Ehsan, H

Reichheld, JP

Roef, L

Witters, E

Lardon, F

Van Bockstaele, D

Van Montagu, M

Inzé, D

Van Onckelen, H

机构：

[1] Univ Instelling Antwerp, Dept Biol, Lab Plantenbiochem & Fysiol, B-2610 Antwerp, Belgium

[2] Univ Instelling Antwerp, UZA, Lab Expt Hematol, B-2650 Edegem, Belgium

[3] State Univ Ghent VIB, Dept Genet, Genet Lab, B-9000 Ghent, Belgium

[4] Univ Ghent, Inst Natl Rech Agron, B-9000 Ghent, Belgium

来源：

FEBS LETTERS | 1998年 / 422卷 / 02期

关键词：

cell cycle; adenosine; 3; 5 '-cyclic monophosphate; indomethacin; mass spectrometry; synchronisation; tobacco BY-2 cell;

D O I：

10.1016/S0014-5793(97)01610-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The evolution of adenosine 3',5'-cyclic monophosphate (cAMP) levels was investigated in synchronised tobacco BY-2 cells by virtue of a method based on immunoaffinity purification and analysis on electrospray tandem mass spectrometry. A transient peak in cAMP content was observed during the S and G(1) phases of the cell cycle. Application of the prostaglandin inhibiting drug indomethacin at early S phase resulted in the loss of the cAMP peak in S phase and inhibited mitotic division. This inhibition of cAMP accumulation suggests the presence of a prostaglandin-dependent adenylyl cyclase activity, analogous to animal cyclases. A potential role for cAMP during the plant cell cycle is postulated. (C) 1998 Federation of European Biochemical Societies.

引用

页码：165 / 169

页数：5

共 37 条

[1] CYCLIC-AMP AS A 2ND MESSENGER IN HIGHER-PLANTS - STATUS AND FUTURE-PROSPECTS [J].