Intein-mediated purification of a recombinantly expressed peptide

被引：12

作者：

Pezza, JA

Allen, KN

Tolan, DR

机构：

[1] Boston Univ, Dept Biol, Boston, MA 02215 USA

[2] Boston Univ, Sch Med, Dept Physiol & Biophys, Boston, MA 02118 USA

来源：

CHEMICAL COMMUNICATIONS | 2004年 / 21期

关键词：

D O I：

10.1039/b407924h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A 26 amino acid peptide has successfully been purified via recombinant expression as an intein fusion protein accompanied by cleavage without the need for any exogenous proteases or cofactors, thus offering a practical, inexpensive approach to produce isotopically labelled peptides.

引用

收藏

页码：2412 / 2413

页数：2

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