The ATP synthase atpHAGDC (F1) operon from Rhodobacter capsulatus

被引：15

作者：

Borghese, R ^{[1
]}

Crimi, M ^{[1
]}

Fava, L ^{[1
]}

Melandri, BA ^{[1
]}

机构：

[1] Univ Bologna, Dept Biol, Biochem & Biophys Lab, I-40126 Bologna, Italy

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 02期

关键词：

D O I：

10.1128/JB.180.2.416-421.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The atpNAGDC operon of Rhodobacter capsulatus, containing the five genes coding for the F-1 sector of the ATP synthase, has been cloned and sequenced. The promoter region has been defined by primer extension analysis. It was not possible to obtain viable cells carrying atp deletions in the R. capsulatus chromosome, indicating that genes coding for ATP synthase are essential, at least under the growth conditions tested. We were able to circumvent this problem by combining gene transfer agent transduction with conjugation. This method represents an easy way to construct strains carrying mutations in indispensable genes.

引用

页码：416 / 421

页数：6

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