The Effect of Epigallocatechin Gallate on Lipopolysaccharide-Induced Acute Lung Injury in a Murine Model

被引：74

作者：

Bae, Hong-Beom ^{[1
,2
]}

Li, Mei ^{[1
,2
,3
]}

Kim, Jong-Phil ^{[1
,2
]}

Kim, Seok-Jai ^{[1
,2
]}

Jeong, Cheol-Won ^{[1
,2
]}

Lee, Hyung-Gon ^{[1
,2
]}

Kim, Woong-Mo ^{[1
,2
]}

Kim, Hyung-Seok ^{[4
]}

Kwak, Sang-Hyun ^{[1
,2
]}

机构：

[1] Chonnam Natl Univ, Dept Anesthesiol, Sch Med, Kwangju 501746, South Korea

[2] Chonnam Natl Univ, Dept Pain Med, Sch Med, Kwangju 501746, South Korea

[3] Chonnam Natl Univ, Brain Korea Project 21, Ctr Biomed Human Resources, Kwangju 501746, South Korea

[4] Chonnam Natl Univ, Dept Forens Med, Sch Med, Kwangju 501746, South Korea

来源：

INFLAMMATION | 2010年 / 33卷 / 02期

关键词：

acute lung injury; cytokine; EGCG; mitogen activated protein kinases; RESPIRATORY-DISTRESS-SYNDROME; TUMOR-NECROSIS-FACTOR; MACROPHAGE INFLAMMATORY PROTEIN-2; GREEN TEA POLYPHENOLS; BETA-GAMMA-SUBUNITS; NF-KAPPA-B; C-X-C; TNF-ALPHA; IN-VIVO; MEDICAL PROGRESS;

D O I：

10.1007/s10753-009-9161-z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

This study was performed to evaluate the effects of epigallocatechin 3 gallate (EGCG) on lipopolysaccharide (LPS)-induced acute lung injury in a murine model. In the present study, production of TNF-alpha and MIP-2 and activation of extracellular signal-regulated kinases (ERK) 1/2, c-Jun amino terminal kinases (JNK) and p38 in RAW264.7 cells were measured. EGCG inhibited the production of TNF-alpha and MIP-2, and attenuated phosphorylation levels of ERK1/2 and JNK, but not p38 in RAW264.7 cells stimulated with LPS. Also, EGCG attenuated the production of TNF-alpha and MIP-2, and the phosphorylation of ERK1/2 and JNK in the lungs of mice administered with LPS intratracheally. It reduced wet/dry weight ratio, histological severities, and neutrophil accumulation in the lungs in mice given LPS. Our results showed that EGCG attenuated LPS-induced lung injury by suppression of the MIP-2 and TNF-alpha production, and ERK1/2 and JNK activation in macrophage stimulated with LPS.

引用

页码：82 / 91

页数：10

共 52 条

[1]

Bacterial endotoxin stimulates macrophages to release HMGB1 partly through CD14- and TNF-dependent mechanisms [J].