The role of histidine-114 of Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase in chain-length determination

被引：6

作者：

Hirooka, K ^{[1
]}

Kato, T ^{[1
]}

Matsu-ura, J ^{[1
]}

Hemmi, H ^{[1
]}

Nishino, T ^{[1
]}

机构：

[1] Tohoku Univ, Dept Biochem & Engn, Aoba Ku, Sendai, Miyagi 9818579, Japan

来源：

FEBS LETTERS | 2000年 / 481卷 / 01期

关键词：

prenyltransferase; geranylgeranyl diphosphate; product specificity; site-directed mutagenesis; molecular modeling;

D O I：

10.1016/S0014-5793(00)01972-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Sulfolobus acidocaldarius geranylgeranyl diphosphate synthase yields (all-E)-C-20 prenyl diphosphate as a final product. The three-dimensional model of the enzyme suggested that removing two bulky residues at 77 and 114 would allow additional prenyl-chain elongation. To test this, we examined several mutants with substitutions at 77 and/or 114. As a result, the mutants, F77G, F77G and H114A, F77G and H114G, H114A. and H114 gave C-30, C-45, C-50, C-30 and C-40 as the main long product, respectively. These observations indicate that histidine-114 plays a crucial role in chain-length determination along with phenylalanine-77. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：68 / 72

页数：5

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