An optimized protocol of a human-to-cattle interspecies somatic cell nuclear transfer

被引：21

作者：

Chang, KH

Lim, JM ^{[1
]}

Kang, SK

Lee, BC

Moon, SY

Hwang, WS

机构：

[1] Seoul Natl Univ, Sch Agr Biotechnol, Seoul 151742, South Korea

[2] Seoul Natl Univ, Coll Vet Med, Seoul 151742, South Korea

[3] Seoul Natl Univ, Coll Med, Med Res Ctr, Seoul 151742, South Korea

[4] Stem Cell Res Ctr, Seoul, South Korea

来源：

FERTILITY AND STERILITY | 2004年 / 82卷 / 04期

关键词：

D O I：

10.1016/j.fertnstert.2004.03.052

中图分类号：

R71 [妇产科学];

学科分类号：

100211 [妇产科学];

摘要：

Human somatic cells were transferred into cattle enucleated oocytes, and a prospective, randomized study was designed to optimize donor cell preparation, fusion medium, and culture method. As a result, improved development of interspecies embryos was achieved by employing serum-starved cord fibroblasts, with Ca2+/Mg2+-free fusion medium and a serum-free medium being used for bovine embryos. (C) 2004 by American Society for Reproductive Medicine.

引用

页码：960 / 962

页数：3

共 16 条

[1]

PHYLOGENY, ONTOGENY AND EMBRYO-TRANSFER [J].