Single 8-oxo-guanine and 8-oxo-adenine lesions induce marked changes in the backbone structure of a 25-base DNA strand

被引:58
作者
Malins, DC
Polissar, NL
Ostrander, GK
Vinson, MA
机构
[1] Pacific NW Res Inst, Mol Epidemiol Program, Seattle, WA 98122 USA
[2] Mt Whisper Light Stat Consulting, Seattle, WA 98112 USA
[3] Univ Washington, Dept Biostat, Seattle, WA 98195 USA
[4] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA
[5] Johns Hopkins Univ, Div Comparat Med, Baltimore, MD 21218 USA
关键词
Fourier transform-infrared spectroscopy; DNA replication; DNA repair; DNA polymerases;
D O I
10.1073/pnas.230438797
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Structural changes in a 25-base DNA strand, induced by single 8-oxo-guanine or 8-oxo-adenine substitutions, were shown by using Fourier transform-infrared spectroscopy with multivariate statistics. Pronounced differences were demonstrated between the parent and derivatives with respect to base interactions and changes in the phospho-deoxyribose backbone. The greatest degree of change in the backbone likely occurred immediately adjacent to the 8-oxo group, potentially altering the stereochemistry at a distance. The 8-oxo lesions, formed from reactive oxygen species (e.g.. hydroxyl radicals), may appreciably alter the conformational properties of strands at the replication fork, thus affecting the selectivity of polymerases. the proofreading capability of repair enzymes, and the fidelity of the transcriptional machinery.
引用
收藏
页码:12442 / 12445
页数:4
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