Molecular and ultrastructural profiles of the symbionts in Cephalotes ants

被引：3

作者：

Bution, M. L. ^{[1
]}

Bresil, C. ^{[1
]}

Destefano, R. H. R. ^{[1
]}

Tango, M. F. de A. ^{[4
]}

da Silveira, W. D. ^{[1
]}

Paulino, L. C. ^{[3
]}

Caetano, F. H. ^{[2
]}

Solferini, V. N. ^{[1
]}

机构：

[1] Univ Estadual Campinas, Dept Genet Evolucao & Bioagentes, Inst Biol, BR-13083970 Campinas, SP, Brazil

[2] Univ Estadual Paulista, Dept Biol, BR-13506900 Rio Claro, SP, Brazil

[3] Univ Fed ABC, Ctr Ciencias Nat & Humans, BR-09090400 Santo Andre, Brazil

[4] Ctr Univ Barao Maua, BR-14090180 Ribeirao Preto, SP, Brazil

来源：

MICRON | 2010年 / 41卷 / 05期

基金：

巴西圣保罗研究基金会;

关键词：

Bacteria; DGGE; PCR; 16S rDNA; TEM; BACTERIA;

D O I：

10.1016/j.micron.2010.01.011

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

The molecular and ultrastructural profiles of the symbionts found in the midgut and ileum of Cephalotes atratus, Cephalotes clypeatus, and Cephalotes pusillus were determined using the V3 region of the bacterial 16S rDNA gene and transmission electron microscopy (T.E.M.). Two samples of C atratus, three of C clypeatus, and six of C. pusillus were analyzed. The coefficients of similarity ranged from 80% to 94% for the samples of symbionts from C. clypeatus and C. atratus, despite being collected in geographically distant sites. The variability within symbionts found in the samples of C. pusillus varied from 29% to 55%, in samples geographically close as well as distant. PCR-DGGE was effective for the purpose of this study and can be considered a versatile tool to analyze gut microbiota. Details of the ultrastructural aspect of these bacteria are presented. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：484 / 489

页数：6

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