Rapid screening method for analyzing the conjugated linoleic acid production capabilities of bacterial cultures

被引:81
作者
Barrett, E.
Ross, R. P.
Fitzgerald, G. F.
Stanton, C. [1 ]
机构
[1] Moorepk Food Res Ctr, TEAGASC, Ctr Biotechnol, Fermoy, Cork, Ireland
[2] Natl Univ Ireland Univ Coll Cork, Biosci Inst, Alimentary Pharmabiot Ctr, Cork, Ireland
[3] Natl Univ Ireland Univ Coll Cork, Dept Microbiol, Cork, Ireland
关键词
D O I
10.1128/AEM.01855-06
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
In this paper we describe a rapid method for identifying bacteria which convert free linoleic acid to conjugated linoleic acid (CLA). This method is based on spectrophotometric detection of CIA and compares well with the standard gas-liquid chromatography method. This method should facilitate high-throughput screening of bacterial isolates for the ability to produce conjugated fatty acids.
引用
收藏
页码:2333 / 2337
页数:5
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