Induction of antigen-specific antitumor immunity with adenovirus-transduced dendritic cells

被引：103

作者：

Gong, J ^{[1
]}

Chen, L ^{[1
]}

Chen, D ^{[1
]}

Kashiwaba, M ^{[1
]}

Manome, Y ^{[1
]}

Tanaka, T ^{[1
]}

Kufe, D ^{[1
]}

机构：

[1] HARVARD UNIV,SCH MED,DANA FARBER CANC INST,DIV CANC PHARMACOL,BOSTON,MA 02115

来源：

GENE THERAPY | 1997年 / 4卷 / 10期

关键词：

dendritic cells; adenovirus; transduction; antitumor immunity; DF3/MUC1;

D O I：

10.1038/sj.gt.3300496

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Transduction of dendritic cells (DC) can result in presentation of tumor-associated antigens and induction of immunity against undefined epitopes. The present studies demonstrate adenovirus (Ad)-mediated transduction of the beta-galactosidase gene in mouse DC. Similar transductions have been obtained with the gene encoding the DF3/MUC1 tumor-associated antigen. We show that the Ad-transduced DC are functional in primary allogeneic mixed lymphocyte reactions. Mice immunized with Ad-transduced DC develop cytotoxic T lymphocytes that are specific for the beta-galactosidase or DF3/MUC1 antigens. The results also demonstrate that Ad.MUC1-transduced DC induce a specific response which inhibits the growth of DF3/MUC1-positive tumors. These findings support the usefulness of Ad-transduced DC for in vivo immunization against tumor-associated antigens.

引用

页码：1023 / 1028

页数：6

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