PCR-RFLP analysis of mitochondrial DNA for identification of snail meat species

被引：78

作者：

Borgo, R ^{[1
]}

SoutyGrosset, C ^{[1
]}

Bouchon, D ^{[1
]}

Gomot, L ^{[1
]}

机构：

[1] UNIV POITIERS,LAB BIOL ANIM,CNRS URA 1975,F-86022 POITIERS,FRANCE

来源：

JOURNAL OF FOOD SCIENCE | 1996年 / 61卷 / 01期

关键词：

mitochondria; land snails; DNA; ribosomal-RNA; genes;

D O I：

10.1111/j.1365-2621.1996.tb14712.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Several species of land snails, including Helix pomatia and Helix lucorum are consumed as food products. The main source of commercial competition is an imported African snail, Achatina fulica. The only way to distinguish between these species has been morphologically. We hypothesized a reliable method for identifying canned snails could be based on using PCR-RFLP analysis of mitochondrial DNA. The molecular weights of the amplified fragments were perfectly identical, regardless of low extraction (fresh snails or cooked and canned samples). The whole amplified products (16S rRNA and 12S rRNA) made it possible to check any fraudulent label and identify the three species using four restriction enzymes (RFLP).

引用

页码：1 / 4

页数：4

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