Efficient in vitro amplification of chronic wasting disease PrpRES

被引：71

作者：

Kurt, Timothy D.

Perrott, Matthew R.

Wilusz, Carol J.

Wilusz, Jeffrey

Supattapone, Surachai

Telling, Glenn C.

Zabel, Mark D.

Hoover, Edward A. ^{[1
]}

机构：

[1] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

[2] Dartmouth Coll, Hitchcock Med Ctr, Dartmouth Med Sch, Dept Biochem, Hanover, NH 03756 USA

[3] Univ Kentucky, Dept Mol Biol & Genet, Lexington, KY USA

来源：

JOURNAL OF VIROLOGY | 2007年 / 81卷 / 17期

关键词：

D O I：

10.1128/JVI.00635-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Chronic wasting disease (CWD) of cervids is associated with conversion of the normal cervid prion protein, PrPC, to a protease-resistant conformer, PrPCWD. Here we report the use of both nondenaturing amplification and protein-misfolding cyclic amplification (PMCA) to amplify PrPCWD in vitro. Normal brains from deer, transgenic mice expressing cervid PrPC [Tg(cerPrP)1536 mice], and ferrets supported amplification. PMCA using normal Tg(cerPrP)1536 brains as the PrPC substrate produced > 6.5 X 10(9)-fold amplification after six rounds. Highly efficient in vitro amplification of PrPCWD is a significant step toward detection of PrPCWD in the body fluids or excreta of CWD-susceptible species.

引用

页码：9605 / 9608

页数：4

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