Determination of prednisolone and the most important associated compounds in ocular and cutaneous pharmaceutical preparations by micellar electrokinetic capillary chromatography

被引:33
作者
Gallego, JML [1 ]
Arroyo, JP [1 ]
机构
[1] Univ Castilla La Mancha, Fac Ciencias Quim, Dept Analyt Chem & Food Technol, E-13071 Ciudad Real, Spain
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2003年 / 784卷 / 01期
关键词
prednisolone; naphazoline; bacitracin; Zn; sulfacetamide; phenylefrine;
D O I
10.1016/S1570-0232(02)00748-1
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A micellar electrokinetic capillary chromatographic method to separate prednisolone, prednisolone acetate, naphazoline, Zn-bacitracin, sulfacetamide and phenylefrine is described. The separation was carried out by using a fused-silica capillary (57 cm x 75 mum I.D.) at 25degreesC and 30 kV, using a 5 mM phosphate-5 mM borate buffer adjusted to pH 8.2, 50 mM sodium dodecyl sulfate (SDS) and 10% methanol-water (v/v) as background electrolyte. Under these conditions, the run time was 8 min and the limits of quantification were about 1.0 mg/l for every component. The method was applied to pharmaceutical preparations and the results provided recoveries close to 100% and the method gave good results when compared with a reference multivariate calibration spectrophotometric method. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:39 / 47
页数:9
相关论文
共 40 条
[1]  
Abdel-Salam M., 1986, ANAL LETT, V19, P2207, DOI [10.1080/00032718608064547, DOI 10.1080/00032718608064547]
[2]   QUANTITATION OF CORTICOSTEROIDS AS COMMON ADULTERANTS IN LOCAL-DRUGS BY HPLC [J].
AHMED, S ;
RIAZ, M .
CHROMATOGRAPHIA, 1991, 31 (1-2) :67-70
[3]   APPROACHES TO OPTIMIZATION OF PRECISION IN CAPILLARY ELECTROPHORESIS [J].
ALTRIA, KD ;
FABRE, H .
CHROMATOGRAPHIA, 1995, 40 (5-6) :313-320
[4]   VALIDATION OF A CAPILLARY ELECTROPHORESIS METHOD FOR THE DETERMINATION OF A QUINOLONE ANTIBIOTIC AND ITS RELATED IMPURITIES [J].
ALTRIA, KD ;
CHANTER, YL .
JOURNAL OF CHROMATOGRAPHY A, 1993, 652 (02) :459-463
[5]  
BOCIC R, 1992, J AOAC INT, V75, P902
[6]   Identification of ten corticosteroids in human hair by liquid chromatography-ionspray mass spectrometry [J].
Cirimele, V ;
Kintz, P ;
Dumestre, V ;
Goullé, JP ;
Ludes, B .
FORENSIC SCIENCE INTERNATIONAL, 2000, 107 (1-3) :381-388
[7]   Electrochemically modulated liquid chromatography coupled on-line with electrospray mass spectrometry [J].
Deng, HT ;
Van Berkel, OJ .
ANALYTICAL CHEMISTRY, 2000, 72 (11) :2641-2647
[8]   SIMULTANEOUS DETERMINATION OF EPHEDRINE AND 2-IMIDAZOLINES IN PHARMACEUTICAL FORMULATIONS BY REVERSED-PHASE HPLC [J].
DEORSI, D ;
GAGLIARDI, L ;
CAVAZZUTTI, G ;
MEDIATI, MG ;
TONELLI, D .
JOURNAL OF LIQUID CHROMATOGRAPHY, 1995, 18 (16) :3233-3242
[9]   Simultaneous high performance liquid chromatographic and derivative ratio spectra spectrophotometry determination of chlorpheniramine maleate and phenylephrine hydrochloride [J].
Erk, N ;
Kartal, M .
FARMACO, 1998, 53 (8-9) :617-622
[10]  
GALLAGHER JB, 1982, J ASSOC OFF ANA CHEM, V65, P1178