A spinosyn-sensitive Drosophila melanogaster nicotinic acetylcholine receptor identified through chemically induced target site resistance, resistance gene identification, and heterologous expression

被引：120

作者：

Watson, Gerald B. ^{[1
]}

Chouinard, Scott W. ^{[2
]}

Cook, Kevin R. ^{[3
]}

Geng, Chaoxian ^{[1
,4
]}

Gifford, Jim M. ^{[1
]}

Gustafson, Gary D. ^{[1
]}

Hasler, James M. ^{[1
]}

Larrinua, Ignacio M. ^{[1
]}

Letherer, Ted J. ^{[1
]}

Mitchell, Jon C. ^{[1
]}

Pak, William L. ^{[4
]}

Salgado, Vincent L. ^{[1
]}

Sparks, Thomas C. ^{[1
]}

Stilwell, Geoff E. ^{[2
]}

机构：

[1] Dow AgroSci LLC, Discovery Res, Indianapolis, IN 46268 USA

[2] Cambria Pharmaceut, Woburn, MA 01801 USA

[3] Indiana Univ, Dept Biol, Bloomington Drosophila Stock Ctr, Bloomington, IN 47405 USA

[4] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA

来源：

INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY | 2010年 / 40卷 / 05期

基金：

美国国家卫生研究院;

关键词：

Spinosad; Spinetoram; Spinosyn; Resistance; Drosophila melanogaster; Nicotinic acetylcholine receptor (nAChR); Xeno pus laevis oocytes; Expression; Mode of action; ALPHA-SUBUNIT; D-ALPHA-6; BINDING; MODE; CLONING;

D O I：

10.1016/j.ibmb.2009.11.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Strains of Drosophila melanogaster with resistance to the insecticides spinosyn A, spinosad, and spinetoram were produced by chemical mutagenesis. These spinosyn-resistant strains were not cross-resistant to other insecticides. The two strains that were initially characterized were subsequently found to have mutations in the gene encoding the nicotinic acetylcholine receptor (nAChR) subunit D alpha 6 Subsequently, additional spinosyn-resistant alleles were generated by chemical mutagenesis and were also found to have mutations in the gene encoding D alpha 6, providing convincing evidence that D alpha 6 is a target site for the spinosyns in D. melanogaster. Although a spinosyn-sensitive receptor could not be generated in Xenopus laevis oocytes simply by expressing D alpha 6 alone, co-expression of D alpha 6 with an additional nAChR subunit, D alpha 5, and the chaperone protein ric-3 resulted in an acetylcholine- and spinosyn-sensitive receptor with the pharmacological properties anticipated for a native nAChR. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：376 / 384

页数：9

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