Molecular heterogeneity of the L-1 metallo-β-lactamase family from Stenotrophomonas maltophilia

被引：31

作者：

Sanschagrin, F ^{[1
]}

Dufresne, J ^{[1
]}

Levesque, RC ^{[1
]}

机构：

[1] Univ Laval, Fac Med, St Foy, PQ G1K 7P4, Canada

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 1998年 / 42卷 / 05期

关键词：

D O I：

10.1128/AAC.42.5.1245

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have determined the nucleotide sequence of the blaS gene encoding the carbapenem-hydrolyzing L-l beta-lactamase from Stenotrophomonas maltophilia GN12873. Analysis of the DNA and deduced amino acid sequences identified a product of 290 amino acids. Comparisons of the L-l amino acid sequence with those of other zinc beta-lactamases showed 88.6% identify with the L-1 enzyme from S. maltophilia IID1275 and less than 20% identity with other class B metalloenzymes.

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页码：1245 / 1248

页数：4

相关论文

共 18 条

[1] THE PRODUCTION AND MOLECULAR-PROPERTIES OF THE ZINC BETA-LACTAMASE OF PSEUDOMONAS-MALTOPHILIA IID-1275 [J].

BICKNELL, R ;

EMANUEL, EL ;

GAGNON, J ;

WALEY, SG .

BIOCHEMICAL JOURNAL, 1985, 229 (03) :791-797

[2] THE FOCUSING POSITIONS OF POLYPEPTIDES IN IMMOBILIZED PH GRADIENTS CAN BE PREDICTED FROM THEIR AMINO-ACID-SEQUENCES [J].

BJELLQVIST, B ;

HUGHES, GJ ;

PASQUALI, C ;

PAQUET, N ;

RAVIER, F ;

SANCHEZ, JC ;

FRUTIGER, S ;

HOCHSTRASSER, D .

ELECTROPHORESIS, 1993, 14 (10) :1023-1031

[3] THE 3-D STRUCTURE OF A ZINC METALLO-BETA-LACTAMASE FROM BACILLUS-CEREUS REVEALS A NEW-TYPE OF PROTEIN FOLD [J].

CARFI, A ;

PARES, S ;

DUEE, E ;

GALLENI, M ;

DUEZ, C ;

FRERE, JM ;

DIDEBERG, O .

EMBO JOURNAL, 1995, 14 (20) :4914-4921

[4] Crystal structure of the wide-spectrum binuclear zinc beta-lactamase from Bacteroides fragilis [J].

Concha, NO ;

Rasmussen, BA ;

Bush, K ;

Herzberg, O .

STRUCTURE, 1996, 4 (07) :823-836

[5] CLONING AND EXPRESSION OF THE IMIPENEM-HYDROLYZING BETA-LACTAMASE OPERON FROM PSEUDOMONAS-MALTOPHILIA IN ESCHERICHIA-COLI [J].

DUFRESNE, J ;

VEZINA, G ;

LEVESQUE, RC .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (06) :819-826

[6] CLONING AND SEQUENCING OF THE METALLOTHIOPROTEIN BETA-LACTAMASE-II GENE OF BACILLUS-CEREUS 569/H IN ESCHERICHIA-COLI [J].

HUSSAIN, M ;

CARLINO, A ;

MADONNA, MJ ;

LAMPEN, JO .

JOURNAL OF BACTERIOLOGY, 1985, 164 (01) :223-229

[7]

KATO C, 1985, J GEN MICROBIOL, V131, P3317

[8] CLONING, NUCLEOTIDE-SEQUENCE, AND EXPRESSION OF THE BACILLUS-CEREUS 5/B/6 BETA-LACTAMASE-II STRUCTURAL GENE [J].

LIM, HM ;

PENE, JJ ;

SHAW, RW .

JOURNAL OF BACTERIOLOGY, 1988, 170 (06) :2873-2878

[9] THE AEROMONAS-HYDROPHILA CPHA GENE - MOLECULAR HETEROGENEITY AMONG CLASS-B METALLO-BETA-LACTAMASES [J].

MASSIDDA, O ;

ROSSOLINI, GM ;

SATTA, G .

JOURNAL OF BACTERIOLOGY, 1991, 173 (15) :4611-4617

[10] MOLECULAR CHARACTERIZATION OF AN ENTEROBACTERIAL METALLO BETA-LACTAMASE FOUND IN A CLINICAL ISOLATE OF SERRATIA-MARCESCENS THAT SHOWS IMIPENEM RESISTANCE [J].

OSANO, E ;

ARAKAWA, Y ;

WACHAROTAYANKUN, R ;

OHTA, M ;

HORII, T ;

ITO, H ;

YOSHIMURA, F ;

KATO, N .

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (01) :71-78