Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan Probe

被引：72

作者：

Reischer, GH

Lemmens, M

Farnleitner, A

Adler, A

Mach, RL

机构：

[1] Tech Univ Vienna, Inst Chem Engn, Gene Technol Grp, A-1060 Vienna, Austria

[2] IFA Tulln, Inst Agrobiotechnol, Dept Plant Protect, A-3430 Tulln, Austria

[3] Austrian Agcy Hlth & Food Safety, Inst Agr Anal, A-4021 Linz, Austria

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 59卷 / 01期

关键词：

Fusarium graminearum; infected wheat; real-time PCR; TaqMan probe;

D O I：

10.1016/j.mimet.2004.06.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude. (C) 2004 Elsevier B.V. All rights reserved.

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收藏

页码：141 / 146

页数：6

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