Clostridium straminisolvens sp nov., a moderately thermophilic, aerotolerant and cellulolytic bacterium isolated from a cellulose-degrading bacterial community

被引:101
作者
Kato, S
Haruta, S
Cui, ZJ
Ishii, M
Yokota, A
Igarashi, Y
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Biotechnol, Bunkyo Ku, Tokyo 1138657, Japan
[2] Univ Tokyo, Inst Mol & Cellular Biosci, Lab Bioresources, Bunkyo Ku, Tokyo 1138657, Japan
关键词
D O I
10.1099/ijs.0.63148-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A novel anaerobic, thermophilic and cellulolytic bacterium (strain CSK1(T)) was isolated from a cellulose-degrading bacterial community. On the basis of 16S rRNA gene sequence similarity, strain CSK1(T) was mapped to cluster III of the genus Clostridium. Strain CSK1(T) is closely related to Clostridium thermocellum (96.2%) and Clostridium aldrichii (95.1%). Strain CSK1(T) is a non-motile, spore-forming, straight or slightly curved rod. The optimum temperature and initial pH for its growth and cellulose degradation are 50-55degreesC and pH 7.5. Strain CSK1(T) grew under a gas phase containing up to 4% O-2. Phylogenetic and phenotypic analyses support the differentiation of strain CSK1(T) from its closest relatives. Strain CSK1(T) therefore represents a novel species, for which the name Clostridium straminisolvens sp. nov. is proposed, with CSK1(T) ( = DSM 16021(T)= IAM 15070(T)) as the type strain.
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页码:2043 / 2047
页数:5
相关论文
共 27 条
[1]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[2]  
[Anonymous], 1995, MOL MICROBIAL ECOLOG
[3]   THE PHYLOGENY OF THE GENUS CLOSTRIDIUM - PROPOSAL OF 5 NEW GENERA AND 11 NEW SPECIES COMBINATIONS [J].
COLLINS, MD ;
LAWSON, PA ;
WILLEMS, A ;
CORDOBA, JJ ;
FERNANDEZGARAYZABAL, J ;
GARCIA, P ;
CAI, J ;
HIPPE, H ;
FARROW, JAE .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1994, 44 (04) :812-826
[4]  
Coughlan M. P., 1992, The prokaryotes: a handbook on the biology of bacteria: ecophysiology, isolation, identification, applications, vol.I., P460
[5]   FERMENTATION OF INULIN BY CLOSTRIDIUM-THERMOSUCCINOGENES SP-NOV, A THERMOPHILIC ANAEROBIC BACTERIUM ISOLATED FROM VARIOUS HABITATS [J].
DRENT, WJ ;
LAHPOR, GA ;
WIEGANT, WM ;
GOTTSCHAL, JC .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (02) :455-462
[6]   FLUOROMETRIC DEOXYRIBONUCLEIC ACID DEOXYRIBONUCLEIC ACID HYBRIDIZATION IN MICRODILUTION WELLS AS AN ALTERNATIVE TO MEMBRANE-FILTER HYBRIDIZATION IN WHICH RADIOISOTOPES ARE USED TO DETERMINE GENETIC RELATEDNESS AMONG BACTERIAL STRAINS [J].
EZAKI, T ;
HASHIMOTO, Y ;
YABUUCHI, E .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1989, 39 (03) :224-229
[7]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[8]   THE 3RD CELLULASE OF ALKALOPHILIC BACILLUS SP STRAIN N-4 - EVOLUTIONARY RELATIONSHIPS WITHIN THE CEL GENE FAMILY [J].
FUKUMORI, F ;
KUDO, T ;
SASHIHARA, N ;
NAGATA, Y ;
ITO, K ;
HORIKOSHI, K .
GENE, 1989, 76 (02) :289-298
[9]   Construction of a stable microbial community with high cellulose-degradation ability [J].
Haruta, S ;
Cui, Z ;
Huang, Z ;
Li, M ;
Ishii, M ;
Igarashi, Y .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2002, 59 (4-5) :529-534
[10]   Nucleotide sequences of two contiguous and highly homologous xylanase genes xynA and xynB and characterization of XynA from Clostridium thermocellum [J].
Hayashi, H ;
Takehara, M ;
Hattori, T ;
Kimura, T ;
Karita, S ;
Sakka, K ;
Ohmiya, K .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1999, 51 (03) :348-357