Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR

被引：31

作者：

Bialasiewicz, Seweryn

Whiley, David M.

Nissen, Michael D.

Sloots, Theo P.

机构：

[1] Royal Childrens Hosp & Hlth Dist, Sir Albert Sakzewski Virus Res Ctr, Queensland Paediat Infect Dis Lab, Herston, Qld 4029, Australia

[2] Univ Queensland, Clin Med Virol Ctr, Brisbane, Qld, Australia

[3] Queensland Hlth PAthol Serv, Div Microbiol, Brisbane, Qld, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2007年 / 45卷 / 05期

关键词：

D O I：

10.1128/JCM.02145-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.

引用

页码：1621 / 1623

页数：3

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