Comparative immunogenicity analysis of modified vaccinia Ankara vectors expressing native or modified forms of hepatitis C virus E1 and E2 glycoproteins

被引：23

作者：

Abraham, JD

Himoudi, N

Kien, F

Berland, JL

Codran, A

Bartosch, B

Baumert, T

Paranhos-Baccala, G

Schuster, C

Inchauspé, G

Kieny, MP

机构：

[1] INSERM, U544, Inst Virol, F-67000 Strasbourg, France

[2] Ecole Normale Super Lyon, CNRS, UMR 2142, BioMerieux, F-69364 Lyon 07, France

[3] CERVI, CNRS, UMR 2142, BioMerieux, F-69364 Lyon 07, France

[4] Ecole Normale Super Lyon, INSERM, U412, IFR 128, F-69364 Lyon 07, France

[5] Univ Freiburg, Dept Med 2, Freiburg, Germany

来源：

VACCINE | 2004年 / 22卷 / 29-30期

关键词：

HCV; MVA; immunization; humoral and cellular responses;

D O I：

10.1016/j.vaccine.2004.04.005

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have evaluated in C57[1316 and HLA-A2.1 transgenic mice the immunogenicity of three MVA vectors expressing either native HCV El E2 polyprotein. truncated and secreted El (E'1 (311)) and E2 (E'2(661)) proteins, or a chimeric El E2 heterodimer presented at the plasma membrane. Immunization induced mainly a Th1 response in HLA-A2.1 transgenic mice while a Th2-type response was detected in C57/B16 mice. Comparison of the three vectors shows an increase in the humoral response when antigens are secreted or membrane bound, and slightly in the cellular response when antigens are exposed on the cell surface. (C) 2004 Elsevier Ltd. All rights reserved.

引用

收藏

页码：3917 / 3928

页数：12

相关论文

共 50 条

[1] Infectious hepatitis C virus pseudo-particles containing functional E1-E2 envelope protein complexes [J].

Bartosch, B ;

Dubuisson, J ;

Cosset, FL .

JOURNAL OF EXPERIMENTAL MEDICINE, 2003, 197 (05) :633-642

[2] Hepatitis C virus structural proteins assemble into viruslike particles in insect cells [J].

Baumert, TF ;

Ito, S ;

Wong, DT ;

Liang, TJ .

JOURNAL OF VIROLOGY, 1998, 72 (05) :3827-3836

[3] Influence of cellular location of expressed antigen on the efficacy of DNA vaccination: cytotoxic T lymphocyte and antibody responses are suboptimal when antigen is cytoplasmic after intramuscular DNA immunization [J].

Boyle, JS ;

Koniaras, C ;

Lew, AM .

INTERNATIONAL IMMUNOLOGY, 1997, 9 (12) :1897-1906

[4] Different hepatitis C virus nonstructural protein 3 (Ns3)-DNA-expressing vaccines induce in HLA-A2.1 transgenic mice stable cytotoxic T lymphocytes that target one major epitope [J].

Brinster, C ;

Muguet, S ;

Lone, YC ;

Boucreux, D ;

Renard, N ;

Fournillier, A ;

Lemonnier, F ;

Inchauspé, G .

HEPATOLOGY, 2001, 34 (06) :1206-1217

[5] Highly attenuated modified vaccinia virus Ankara (MVA) as an effective recombinant vector: A murine tumor model [J].

Carroll, MW ;

Overwijk, WW ;

Chamberlain, RS ;

Rosenberg, SA ;

Moss, B ;

Restifo, NP .

VACCINE, 1997, 15 (04) :387-394

[6] Efficient generation of recombinant adenovirus vectors by homologous recombination in Escherichia coli [J].

Chartier, C ;

Degryse, E ;

Gantzer, M ;

Dieterle, A ;

Pavirani, A ;

Mehtali, M .

JOURNAL OF VIROLOGY, 1996, 70 (07) :4805-4810

[7] ISOLATION OF A CDNA CLONE DERIVED FROM A BLOOD-BORNE NON-A, NON-B VIRAL-HEPATITIS GENOME [J].

CHOO, QL ;

KUO, G ;

WEINER, AJ ;

OVERBY, LR ;

BRADLEY, DW ;

HOUGHTON, M .

SCIENCE, 1989, 244 (4902) :359-362

[8] Characterization of aggregates of hepatitis C virus glycoproteins [J].

Choukhi, A ;

Pillez, A ;

Drobecq, H ;

Sergheraert, C ;

Wychowski, C ;

Dubuisson, J .

JOURNAL OF GENERAL VIROLOGY, 1999, 80 :3099-3107

[9] Charged residues in the transmembrane domains of hepatitis C virus glycoproteins play a major role in the processing, subcellular localization, and assembly of these envelope proteins [J].

Cocquerel, L ;

Wychowski, C ;

Minner, F ;

Penin, F ;

Dubuisson, J .

JOURNAL OF VIROLOGY, 2000, 74 (08) :3623-3633

[10] A retention signal necessary and sufficient for endoplasmic reticulum localization maps to the transmembrane domain of hepatitis C virus glycoprotein E2 [J].

Cocquerel, L ;

Meunier, JC ;

Pillez, A ;

Wychowski, C ;

Dubuisson, J .

JOURNAL OF VIROLOGY, 1998, 72 (03) :2183-2191

← 1 2 3 4 5 →