High-performance liquid chromatographic determination of pioglitazone and its metabolites in human serum and urine

被引：68

作者：

Yamashita, K

Murakami, H

Okuda, T

Motohashi, M

机构：

[1] Drug Anal. Pharmacokin. Res. Labs., Takeda Chemical Industries, Ltd., Osaka 532, Juso-Honmachi, Yodogawa-ku

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1996年 / 677卷 / 01期

关键词：

pioglitazone;

D O I：

10.1016/0378-4347(95)00440-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatographic (HPLC) method for the simultaneous determination of pioglitazone and its metabolites (M-I to M-V) in human serum and urine was developed. The method for serum involved the solid-phase and liquid-liquid extraction. Urine with and without enzymatic hydrolysis using beta-glucuronidase was treated with liquid-liquid extraction. The compounds in the extract were analyzed using HPLC with UV detection at 269 nm. The detection limits of pioglitazone, M-I, M-II, M-III, M-IV and M-V in serum were 0.01-0.05 mu g/ml, those in urine were 0.1-0.5 mu g/ml, and those in urine after enzymatic hydrolysis were 0.3-0.5 mu g/ml, respectively. The method was applied to the clinical trials of pioglitazone.

引用

页码：141 / 146

页数：6

共 13 条

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