Short-term effects of leptin on skeletal muscle protein metabolism in the rat

被引:26
作者
Carbó, N [1 ]
Ribas, V [1 ]
Busquets, S [1 ]
Alvarez, B [1 ]
López-Soriano, FJ [1 ]
Argilés, JM [1 ]
机构
[1] Univ Barcelona, Fac Biol, Dept Bioquim & Biol Mol, E-08071 Barcelona, Spain
关键词
leptin; skeletal muscle; proteolysis; protein turnover; ubiquitin; ATP-dependent proteolysis;
D O I
10.1016/S0955-2863(00)00101-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have examined the short-term effects of leptin on protein metabolism in the rat. Indeed, an intravenous leptin administration (100 mug/kg body weight), which resulted in no changes in circulating insulin in the time interval studied, induced a decrease in the incorporation of C-14-leucine to C-14-skeletal muscle protein. No changes were observed in relation to muscle protein degradation (either measured in vivo following isotope preloading or in vitro as tyrosine released into the incubation medium) and gene expression associated with the different proteolytic systems (cathepsin B, m-calpain and ubiquitin-proteasome system). The effects of leptin on amino acid incorporation into muscle protein do not seem to be direct because incubation of isolated EDL muscles in the presence of 10 mug/ml of leptin did not modify either the protein incorporation or the oxidation of C-14-leucine. It may, therefore, be suggested that leptin is able to influence protein synthesis in skeletal muscle through the action of an unknown mediator. (J. Nutr. Biochem. 11:431-435, 2000) (C) Elsevier Science Inc. 2000 All rights reserved.
引用
收藏
页码:431 / 435
页数:5
相关论文
共 35 条
[1]   UBIQUITIN IS A HEAT-SHOCK PROTEIN IN CHICKEN-EMBRYO FIBROBLASTS [J].
BOND, U ;
SCHLESINGER, MJ .
MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (05) :949-956
[2]  
BOTBOL V, 1991, J BIOL CHEM, V266, P2151
[3]   SINGLE-PULSE SHOCK-TUBE STUDIES OF HYDROCARBON PYROLYSIS .5. PYROLYSIS OF NEOPENTANE [J].
BRADLEY, JN ;
WEST, KO .
JOURNAL OF THE CHEMICAL SOCIETY-FARADAY TRANSACTIONS I, 1976, 72 :8-19
[4]   RECOMBINANT MOUSE OB PROTEIN - EVIDENCE FOR A PERIPHERAL SIGNAL LINKING ADIPOSITY AND CENTRAL NEURAL NETWORKS [J].
CAMPFIELD, LA ;
SMITH, FJ ;
GUISEZ, Y ;
DEVOS, R ;
BURN, P .
SCIENCE, 1995, 269 (5223) :546-549
[5]   TISSUE PROTEIN-TURNOVER DURING LIVER CARCINOGENESIS [J].
CANUTO, RA ;
TESSITORE, L ;
MUZIO, G ;
AUTELLI, R ;
BACCINO, FM .
CARCINOGENESIS, 1993, 14 (12) :2581-2587
[6]   Leptin: The tale of an obesity gene [J].
Caro, JF ;
Sinha, MK ;
Kolaczynski, JW ;
Zhang, PL ;
Considine, RV .
DIABETES, 1996, 45 (11) :1455-1462
[7]   Peripheral metabolic actions of leptin [J].
Cawthorne, MA ;
Morton, NM ;
Pallett, AL ;
Liu, YL ;
Emilsson, V .
PROCEEDINGS OF THE NUTRITION SOCIETY, 1998, 57 (03) :449-453
[8]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[9]   INSULIN-STIMULATED ALPHA-(METHYL)AMINOISOBUTYRIC ACID UPTAKE IN SKELETAL-MUSCLE - EVIDENCE FOR A SHORT-TERM ACTIVATION OF UPTAKE INDEPENDENT OF NA+ ELECTROCHEMICAL GRADIENT AND PROTEIN-SYNTHESIS [J].
GUMA, A ;
TESTAR, X ;
PALACIN, M ;
ZORZANO, A .
BIOCHEMICAL JOURNAL, 1988, 253 (03) :625-629
[10]   Leptin concentrations in diabetic and nondiabetic Mexican-Americans [J].
Haffner, SM ;
Stern, MP ;
Miettinen, H ;
Wei, M ;
Gingerich, RL .
DIABETES, 1996, 45 (06) :822-824