Development of diagnostic concentrations for monitoring Bacillus thuringiensis resistance in European corn borer (Lepidoptera: Crambidae)

被引:50
作者
Marçon, PCRG [1 ]
Siegfried, BD [1 ]
Spencer, T [1 ]
Hutchison, WD [1 ]
机构
[1] Univ Nebraska, Dept Entomol, Lincoln, NE 68583 USA
关键词
European corn borer; Bacillus thuringiensis; resistance monitoring;
D O I
10.1603/0022-0493-93.3.925
中图分类号
Q96 [昆虫学];
学科分类号
摘要
Two candidate diagnostic concentrations of the Cry1Ab and Cry1Ac toxins from Bacillus thuringiensis corresponding to the LC99 and EC99 (effective concentration that causes 99% growth inhibition) for European corn borer, Ostrinia nubilalis (Hubner), were determined based on previously obtained baseline data. Validation experiments using field-collected European corn borer populations from across North America showed that for Cry1Ab, a concentration corresponding to the upper limit of the 95% confidence interval of the LC99, produced mortality >99% for all populations tested. However, for Cry1Ac, adjustments and further validation are probably necessary. Development of B. thuringiensis resistance monitoring programs that rely on diagnostic techniques are discussed.
引用
收藏
页码:925 / 930
页数:6
相关论文
共 30 条
[1]   Using an F2 screen to search for resistance alleles to Bacillus thuringiensis toxin in European corn borer (Lepidoptera: Crambidae) [J].
Andow, DA ;
Alstad, DN ;
Pang, YH ;
Bolin, PC ;
Hutchison, WD .
JOURNAL OF ECONOMIC ENTOMOLOGY, 1998, 91 (03) :579-584
[2]  
[Anonymous], 1983, Statistical methods
[3]   FIELD-EVALUATION OF EUROPEAN CORN-BORER CONTROL IN PROGENY OF 173 TRANSGENIC CORN EVENTS EXPRESSING AN INSECTICIDAL PROTEIN FROM BACILLUS-THURINGIENSIS [J].
ARMSTRONG, CL ;
PARKER, GB ;
PERSHING, JC ;
BROWN, SM ;
SANDERS, PR ;
DUNCAN, DR ;
STONE, T ;
DEAN, DA ;
DEBOER, DL ;
HART, J ;
HOWE, AR ;
MORRISH, FM ;
PAJEAU, ME ;
PETERSEN, WL ;
REICH, BJ ;
RODRIGUEZ, R ;
SANTINO, CG ;
SATE, SJ ;
SCHULER, W ;
SIMS, SR ;
STEHLING, S ;
TAROCHIONE, LJ ;
FROMM, ME .
CROP SCIENCE, 1995, 35 (02) :550-557
[4]  
Bolin PC, 1998, J AGR ENTOMOL, V15, P231
[5]  
BOLIN PC, 1998, THESIS U MINNESOTA S
[6]  
Dennehey T. J., 1987, Combating Resistance to Xenobiotics: biological and chemical approaches, P118
[7]  
Finney D.J., 1977, PROBIT ANAL, VIII
[8]  
Fischhoff David A., 1996, V15, P214
[9]  
French-Constant RH., 1990, PESTICIDE RESISTANCE, P4, DOI [10.1007/978-1-4684-6429-0_2, DOI 10.1007/978-1-4684-6429-0_2]
[10]   SELECTION AND GENETIC-ANALYSIS OF A HELIOTHIS-VIRESCENS (LEPIDOPTERA, NOCTUIDAE) STRAIN WITH HIGH-LEVELS OF RESISTANCE TO BACILLUS-THURINGIENSIS TOXINS [J].
GOULD, F ;
ANDERSON, A ;
REYNOLDS, A ;
BUMGARNER, L ;
MOAR, W .
JOURNAL OF ECONOMIC ENTOMOLOGY, 1995, 88 (06) :1545-1559