Droplets as microreactors for high-throughput biology

被引：112

作者：

Taly, Valerie

Kelly, Bernard T.

Griffiths, Andrew D.

机构：

[1] MRC, Mol Biol Lab, Cambridge CB2 2QH, England

[2] Cambridge Inst Med Res, Cambridge CB2 2XY, England

[3] Univ Strasbourg, CNRS, UMR 7006, ISIS,Lab Biol Chim, F-67083 Strasbourg, France

来源：

CHEMBIOCHEM | 2007年 / 8卷 / 03期

基金：

英国医学研究理事会;

关键词：

directed evolution; high-throughput screening; in-vitro compartmentalization; polymerase chain reaction; IN-VITRO COMPARTMENTALIZATION; SINGLE DNA-MOLECULES; DIRECTED EVOLUTION; PERIPLASMIC EXPRESSION; BINDING PROTEINS; ENZYME LIBRARIES; HUMAN-ANTIBODIES; PHAGE DISPLAY; SELECTION; EMULSION;

D O I：

10.1002/cbic.200600425

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Inspired by the principles of biological evolution, biologists-and others-have in recent decades harnessed the power of "natural" selection to sift through huge libraries of genes and find those with desirable properties. At the same time, the demand for high-throughput biochemical and genetic assays and screens has driven the development of increasingly miniaturised assay Systems. An exciting synergy is now emerging between these two fields, whereby the tools of ultrahigh-throughput screening promise to open up new directions in molecular engineering.

引用

页码：263 / 272

页数：10

共 69 条

[1] Selection of ribozymes that catalyse multiple-turnover Diels-Alder cycloadditions by using in vitro compartmentalization
Agresti, JJ
Kelly, BT
Jäschke, A
Griffiths, AD
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) : 16170 - 16175
[2] High-throughput screening of enzyme libraries: Thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments
Aharoni, A
Amitai, G
Bernath, K
Magdassi, S
Tawfik, DS
[J]. CHEMISTRY & BIOLOGY, 2005, 12 (12): : 1281 - 1289
[3] Ahn K., 2006, APPL PHYS LETT, V88
[4] In vitro display technologies:: novel developments and applications
Amstutz, P
Forrer, P
Zahnd, C
Plückthun, A
[J]. CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (04) : 400 - 405
[5] Formation of dispersions using "flow focusing" in microchannels
Anna, SL
Bontoux, N
Stone, HA
[J]. APPLIED PHYSICS LETTERS, 2003, 82 (03) : 364 - 366
[6] Directed evolution of protein inhibitors of DNA-nucleases by in vitro compartmentalization (IVC) and nano-droplet delivery
Bernath, K
Magdassi, S
Tawfik, DS
[J]. JOURNAL OF MOLECULAR BIOLOGY, 2005, 345 (05) : 1015 - 1026
[7] In vitro compartmentalization by double emulsions: sorting and gene enrichment by fluorescence activated cell sorting
Bernath, K
Hai, MT
Mastrobattista, E
Griffiths, AD
Magdassi, S
Tawfik, DS
[J]. ANALYTICAL BIOCHEMISTRY, 2004, 325 (01) : 151 - 157
[8] Covalent DNA display as a novel tool for directed evolution of proteins in vitro
Bertschinger, J
Neri, D
[J]. PROTEIN ENGINEERING DESIGN & SELECTION, 2004, 17 (09) : 699 - 707
[9] Isolation of high-affinity ligand-binding proteins by periplasmic expression with cytometric screening (PECS)
Chen, G
Hayhurst, A
Thomas, JG
Harvey, BR
Iverson, BL
Georgiou, G
[J]. NATURE BIOTECHNOLOGY, 2001, 19 (06) : 537 - 542
[10] Altering the sequence specificity of HaeIII methyltransferase by directed evolution using in vitro compartmentalization
Cohen, HM
Tawfik, DS
Griffiths, AD
[J]. PROTEIN ENGINEERING DESIGN & SELECTION, 2004, 17 (01) : 3 - 11

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