Arg260-Cys mutation in severe factor XIII deficiency: conformational change of the A subunit is predicted by molecular modelling and mechanics

被引:38
作者
Ichinose, A
Tsukamoto, H
Izumi, T
Yamazaki, T
Togashi, M
Takamatsu, J
Saito, H
Umeyama, K
机构
[1] Yamagata Univ, Sch Med, Dept Mol Pathobiochem, Yamagata 9909585, Japan
[2] Kitasato Univ, Sch Pharmaceut Sci, Dept Chem Phys, Tokyo 108, Japan
[3] Nagoya Univ Hosp, Dept Transfus Med, Nagoya, Aichi, Japan
[4] Nagoya Univ, Sch Med, Dept Med 1, Nagoya, Aichi 466, Japan
关键词
bleeding disorder; factor XIII deficiency; point mutation; molecular modelling; molecular mechanics;
D O I
10.1046/j.1365-2141.1998.00698.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
To explore the implications of the structure/function relationships in factor XIII, a patient with severe A subunit deficiency was examined at the DNA and RNA levels. Nucleotide sequence analysis of the patient's DNA amplified by PCR revealed that the patient had a replacement of C by T in the codon for Arg260. RT-PCR analysis demonstrated that only one kind of mRNA coding for the Arg260-Cys mutation was expressed in the patient at a normal level. Another possible defective allele of the A subunit gene with a G-A polymorphism was not expressed (null allele). The substitution of Arg260 by Cys located on the interface of two A subunits would preclude the reciprocal ionic interaction (salt bridge) between Arg260 and Asp404. Molecular modelling and, for the first time, molecular mechanics calculated that Cys260 changed the local conformation of the A subunit and reduced the electrostatic interaction between two monomers, suggesting destabilization of the molecule's dimer.
引用
收藏
页码:264 / 272
页数:9
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