Influence of Age-Related Degeneration on Regenerative Potential of Human Nucleus Pulposus Cells

被引：29

作者：

Yang, Shu-Hua ^{[3
,4
,5
]}

Lin, Chen-Chiang ^{[3
]}

Hu, Ming-Hsiao ^{[3
]}

Shih, Tiffany Ting-Fang ^{[5
,6
]}

Sun, Yuan-Hui ^{[4
,5
]}

Lin, Feng-Huei ^{[1
,2
]}

机构：

[1] Natl Taiwan Univ, Inst Biomed Engn, Coll Engn, Taipei 10764, Taiwan

[2] Natl Taiwan Univ, Coll Med, Taipei 10764, Taiwan

[3] Natl Taiwan Univ Hosp, Dept Orthopaed, Yun Lin Branch, Douliou City, Yunlin County, Taiwan

[4] Natl Taiwan Univ Hosp, Dept Orthoped, Taipei, Taiwan

[5] Natl Taiwan Univ, Coll Med, Taipei 10764, Taiwan

[6] Natl Taiwan Univ Hosp, Dept Radiol, Taipei, Taiwan

来源：

JOURNAL OF ORTHOPAEDIC RESEARCH | 2010年 / 28卷 / 03期

关键词：

nucleus pulposus; degeneration; regeneration; collagens; TGF-beta; 1; INTERVERTEBRAL DISC CELLS; EXTRACELLULAR-MATRIX; CLINICAL-EXPERIENCE; EXPRESSION; GROWTH; THERAPEUTICS; PHENOTYPE; CULTURE; BACK;

D O I：

10.1002/jor.20988

中图分类号：

R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学（修复外科学）];

学科分类号：

100224 [整形外科学];

摘要：

Nucleus pulposus (NP) cells, sourced from herniation surgeries, may be used as a cell-based therapy for intervertebral disc (IVD) degeneration. But, both the regenerative potential of these degenerative adult NP cells and how to stimulate optimum matrix synthesis is not yet clear. The purpose of the current study was to understand the different phenotypic behaviors between degenerative adult NP cells and normal adolescent NP cells Degenerative adult NP cells produced a significantly higher amount of proteoglycans and collagens than adolescent cells. Insulin-like growth factor-1 was the only anabolic cytokine with increased endogenous expression in degenerative adult NP cells TGF-beta 1 treatment of degenerative NP cells promoted matrix synthesis but stimulated too much type I collagen and suppressed type II collagen and aggrecan. Adult degenerative NP cells possess upregulated regenerative potential, but stimulation in addition to TGF-beta 1 needed to enhance matrix productivity and optimize the collagen expression profile. (C) 2009 Orthopaedic Research Society Published by Wiley Periodicals, Ind J Orthop Res 28 379-383, 2010

引用

页码：379 / 383

页数：5

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