Ferrocenecarboxaldehyde labeled DNA probe for the study on DNA damage and protection

被引：16

作者：

Xu, C ^{[1
]}

Cai, H ^{[1
]}

He, PG ^{[1
]}

Fang, YZ ^{[1
]}

机构：

[1] E China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China

来源：

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY | 2000年 / 367卷 / 06期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1007/s002160000414

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A ferrocenecarboxaldehyde (FCA) labeled DNA probe is used for the first time in the study of DNA damage and protection. The electrochemically active reagent FCA was labeled successfully on to a denatured calf-thymus DNA by 1-ethyl-3- (3-dimethyl-aminopropyl) carbodiimide (EDC). The: FCA labeled DNA probe was used to hybridize with the sample DNA sequence accumulated on the surface of a graphite electrode. The anodic peaks of the FCA bound to the double stranded DNA (dsDNA) by differential pulse voltammetry (DPV) were used for the detection of DNA damage and protection. Thiourea, sodium benzoic acid and isopropanol can decrease DNA damage by hydroxyl radicals, and their protection efficiencies are discussed.

引用

页码：593 / 595

页数：3

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