Regulation of renal Na+,K+-ATPase in rat thick ascending limb during K+ depletion: Evidence for modulation of Na+ affinity

被引:15
作者
BuffinMeyer, B [1 ]
Marsy, S [1 ]
BarletBas, C [1 ]
Cheval, L [1 ]
YounesIbrahim, M [1 ]
Rajerison, R [1 ]
Doucet, A [1 ]
机构
[1] COLL FRANCE,LAB PHYSIOL CELLULAIRE,F-75231 PARIS 5,FRANCE
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1996年 / 490卷 / 03期
关键词
D O I
10.1113/jphysiol.1996.sp021172
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. NaCl reabsorption along the loop of Henle is reduced in K+-depleted rats. Because Na+,K+-ATPase energizes this transport and because K+ depletion is known to induce an upregulation of Na+,K+-ATPase in most tissues, the regulation of this enzyme was investigated at the level of single thick ascending limbs of the loop of Henle freshly microdissected from rats fed either a normal (control rats) or a low-K+ diet (LK rats). 2. Within 2 weeks of K+ depletion, Na+,K+-ATPase activity and [H-3]ouabain binding were increased by 30-50% in the medullary portion of the thick ascending limb (MTAL). 3. Despite this increase in the number of Na+,K+-ATPase units, the transport capacity of the Na+,K+ pump, determined by ouabain-sensitive Rb+ uptake in the presence of an extracellular concentration of Rb+ mimicking the kalaemia determined in control (4.0 mar Rb+) and LK rats (2.3 mM Rb+), was reduced in MTAL from LK rats. 4. Inhibition of the Na+,K+ pump was not accounted for by changes in either extracellular K+ or intracellular Na+ concentrations, but by a decrease in the pump affinity for Na+. 5. Because this change in the apparent affinity of the Na+,K+ pump for Na+ was detectable in intact but not in permeabilized MTAL cells, it is probably induced hy a rapidly; reversible cytosolic factor.
引用
收藏
页码:623 / 632
页数:10
相关论文
共 29 条
[1]   DIFFERENCE IN THE NA AFFINITY OF NA+-K+-ATPASE ALONG THE RABBIT NEPHRON - MODULATION BY K [J].
BARLETBAS, C ;
CHEVAL, L ;
KHADOURI, C ;
MARSY, S ;
DOUCET, A .
AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (02) :F246-F250
[2]   PHOSPHORYLATION OF THE CATALYTIC SUBUNIT OF NA+,K+-ATPASE INHIBITS THE ACTIVITY OF THE ENZYME [J].
BERTORELLO, AM ;
APERIA, A ;
WALAAS, SI ;
NAIRN, AC ;
GREENGARD, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (24) :11359-11362
[3]  
CARROLL MA, 1990, AM J PHYSIOL, V258, pF1084, DOI 10.1152/ajprenal.1990.258.4.F1084
[4]   MEASUREMENT OF NA-K-ATPASE-MEDIATED RUBIDIUM INFLUX IN SINGLE SEGMENTS OF RAT NEPHRON [J].
CHEVAL, L ;
DOUCET, A .
AMERICAN JOURNAL OF PHYSIOLOGY, 1990, 259 (01) :F111-F121
[5]  
CHIBALIN AV, 1992, J BIOL CHEM, V267, P22378
[6]   RENAL CORTEX ION COMPOSITION AND NA-K-ATPASE ACTIVITY IN GENTAMICIN-NEPHROTOXICITY [J].
CRONIN, RE ;
NIX, KL ;
FERGUSON, ER ;
SOUTHERN, PM ;
HENRICH, WL .
AMERICAN JOURNAL OF PHYSIOLOGY, 1982, 242 (05) :F477-F483
[8]   QUANTITATION OF [H-3]OUABAIN BINDING AND TURNOVER OF NA-K-ATPASE ALONG THE RABBIT NEPHRON [J].
ELMERNISSI, G ;
DOUCET, A .
AMERICAN JOURNAL OF PHYSIOLOGY, 1984, 247 (01) :F158-F167
[9]   SITES OF ANTINATRIURETIC ACTION OF INSULIN ALONG RAT NEPHRON [J].
FERAILLE, E ;
MARSY, S ;
CHEVAL, L ;
BARLETBAS, C ;
KHADOURI, C ;
FAVRE, H ;
DOUCET, A .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (01) :F175-F179
[10]   PROTEIN-KINASE C-DEPENDENT STIMULATION OF NA+-K+-ATPASE IN RAT PROXIMAL CONVOLUTED TUBULES [J].
FERAILLE, E ;
CARRANZA, ML ;
BUFFINMEYER, B ;
ROUSSELOT, M ;
DOUCET, A ;
FAVRE, H .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1995, 268 (05) :C1277-C1283