A biological method for the quantitative measurement of tetrodotoxin (TTX): Tissue culture bioassay in combination with a water-soluble tetrazolium salt

被引：66

作者：

Hamasaki, K ^{[1
]}

Kogure, K ^{[1
]}

Ohwada, K ^{[1
]}

机构：

[1] UNIV TOKYO,OCEAN RES INST,NAKANO KU,TOKYO 164,JAPAN

来源：

TOXICON | 1996年 / 34卷 / 04期

关键词：

D O I：

10.1016/0041-0101(95)00151-4

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A tissue culture bioassay, using the mouse neuroblastoma cell line (Neuro2A), was improved to provide a simple and sensitive bioassay for TTX or sodium channel-blocking toxins (SCB). The water-soluble tetrazolium salt, 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2, 4-disulfophenyl)-2H-tetrazolium, monosodium salt (WST-1), was applied to replace the time-consuming and subjective cell-counting procedure of the cells with automatic measurement, using a microplate reader. It was also confirmed that this method is directly applicable to bacterial culture supernatants, with the precaution of possible interference. (C) 1996 Elsevier Science Ltd.

引用

页码：490 / 495

页数：6

共 18 条

[1] SODIUM UPTAKE ASSOCIATED WITH ACTIVATION OF ACTION POTENTIAL IONOPHORES OF CULTURED NEUROBLASTOMA AND MUSCLE-CELLS [J].