The use of FUdR can cause prolonged longevity in mutant nematodes

被引：72

作者：

Aitlhadj, Layla ^{[1
]}

Stuerzenbaum, Stephen R. ^{[1
]}

机构：

[1] Kings Coll London, Div Pharmaceut Sci, Sch Biomed & Hlth Sci, London SE1 9NH, England

来源：

MECHANISMS OF AGEING AND DEVELOPMENT | 2010年 / 131卷 / 05期

基金：

英国医学研究理事会;

关键词：

Lifespan; Longevity; Caenorhabditis elegans; tub-1; FUdR; CAENORHABDITIS-ELEGANS; LIFE-SPAN; STERILIZATION; PATHWAYS; GROWTH;

D O I：

10.1016/j.mad.2010.03.002

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The nematode Caenorhabditis elegans is a popular model organism that is used to study longevity and aging. One drawback of nematode lifespan assays is the labour intensive separation of offspring from adults during the reproductive period. To circumvent this, the worm community frequently adds 5-fluoro-2'-deoxyuridine (FUdR), a drug that induces parental sterility, to the nematode culture. Here, we report that FUdR causes a significant artefactual increase in the longevity of tub-1 mutants which is not observed in wild-type nematodes. This suggests that the effect of FUdR is not neutral and the mechanism of action may result in misleading data, or indeed the misinterpretation of gerontogenes. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

引用

页码：364 / 365

页数：2

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