Probing of bioaffinity interactions at interfaces using impedance spectroscopy and chronopotentiometry

被引：119

作者：

Kharitonov, AB ^{[1
]}

Alfonta, L ^{[1
]}

Katz, E ^{[1
]}

Willner, I ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Inst Chem, IL-91904 Jerusalem, Israel

来源：

JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2000年 / 487卷 / 02期

关键词：

NAD(+)-dependent enzymes; cytochrome c; cytochrome oxidase; impedance spectroscopy; chronopotentiometry; bioaffinity complex;

D O I：

10.1016/S0022-0728(00)00178-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Faradaic impedance spectroscopy and chronopotentiometry are used as electrochemical methods for probing in situ bioaffinity interactions on surfaces between enzymes and their molecular or macromolecular cofactors. Alcohol dehydrogenase (E.C. 1.1.1.1.) and lactate dehydrogenase (E.C. 1.1.1.27) bind to an NAD(+)-functionalized monolayer electrode with association constants corresponding to 1.6 x 10(4) and 1.5 x 10(5) M-1, respectively. Cytochrome oxidase binds to an oriented cytochrome c monolayer assembled on an An electrode with an association constant of K-a= 1.2 x 10(7) M-1. (C) 2000 Elsevier Science S.A. All rights reserved.

引用

页码：133 / 141

页数：9

共 41 条

[1] COVALENT IMMOBILIZATION OF AVIDIN ON GLASSY-CARBON ELECTRODES AS THE BASIS FOR MULTIVALENT BIOSENSORS
ACHTNICH, UR
TIEFENAUER, LX
ANDRES, RY
[J]. BIOSENSORS & BIOELECTRONICS, 1992, 7 (04) : 279 - 290
[2] ALFONTA L, UNPUB BIOSENS BIOELE
[3] Layer-by-layer construction of enzyme multilayers on an electrode for the preparation of glucose and lactate sensors: Elimination of ascorbate interference by means of an ascorbate oxidase multilayer
Anzai, J
Takeshita, H
Kobayashi, Y
Osa, T
Hoshi, T
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (04) : 811 - 817
[4] BARD AJ, 1980, ELECTOCHEMICAL METHO
[5] Sensing and amplification of oligonucleotide-DNA interactions by means of impedance spectroscopy: a route to a Tay-Sachs sensor
Bardea, A
Patolsky, F
Dagan, A
Willner, I
[J]. CHEMICAL COMMUNICATIONS, 1999, (01) : 21 - 22
[6] NAD(+)-dependent enzyme electrodes: Electrical contact of cofactor-dependent enzymes and electrodes
Bardea, A
Katz, E
Buckmann, AF
Willner, I
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1997, 119 (39) : 9114 - 9119
[7] BARDEA A, IN PRESS ELECTROANAL
[8] BOND AM, 1980, POLAROGRAPHIC METHOD
[9] A FULLY ACTIVE MONOLAYER ENZYME ELECTRODE DERIVATIZED BY ANTIGEN-ANTIBODY ATTACHMENT
BOURDILLON, C
DEMAILLE, C
GUERIS, J
MOIROUX, J
SAVEANT, JM
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (26) : 12264 - 12269
[10] MEASUREMENT OF INTERFACIAL PROCESSES AT ELECTRODE SURFACES WITH THE ELECTROCHEMICAL QUARTZ CRYSTAL MICROBALANCE
BUTTRY, DA
WARD, MD
[J]. CHEMICAL REVIEWS, 1992, 92 (06) : 1355 - 1379

← 1 2 3 4 5 →