Global analysis of cell type-specific gene expression

被引：15

作者：

Galbraith, DW ^{[1
]}

机构：

[1] Univ Arizona, Dept Plant Sci, Tucson, AZ 85721 USA

来源：

COMPARATIVE AND FUNCTIONAL GENOMICS | 2003年 / 4卷 / 02期

基金：

美国国家科学基金会;

关键词：

gene expression; GFP; flow cytometry and sorting; eukaryotes; microarrays; high-throughput methods;

D O I：

10.1002/cfg.281

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The tissues and organs of multicellular eukaryotes are frequently observed to comprise complex three-dimensional interspersions of different cell types. It is a reasonable assumption that different global patterns of gene expression are found within these different cell types. This review outlines general experimental strategies designed to characterize these global gene expression patterns, based on a combination of methods of transgenic fluorescent protein (FP) expression and targeting, of flow cytometry and sorting and of high-throughput gene expression analysis. Copyright (C) 2003 John Wiley Sons, Ltd.

引用

页码：208 / 215

页数：8

共 41 条

[1] Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP: Analysis of gene expression during potato tuber development [J].

Bachem, CWB ;

vanderHoeven, RS ;

deBruijn, SM ;

Vreugdenhil, D ;

Zabeau, M ;

Visser, RGF .

PLANT JOURNAL, 1996, 9 (05) :745-753

[2] In vitro cloning of complex mixtures of DNA on microbeads:: Physical separation of differentially expressed cDNAs [J].

Brenner, S ;

Williams, SR ;

Vermaas, EH ;

Storck, T ;

Moon, K ;

McCollum, C ;

Mao, JI ;

Luo, SJ ;

Kirchner, JJ ;

Eletr, S ;

DuBridge, RB ;

Burcham, T ;

Albrecht, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (04) :1665-1670

[3] Gene expression analysis by massively parallel signature sequencing (MPSS) on microbead arrays [J].

Brenner, S ;

Johnson, M ;

Bridgham, J ;

Golda, G ;

Lloyd, DH ;

Johnson, D ;

Luo, SJ ;

McCurdy, S ;

Foy, M ;

Ewan, M ;

Roth, R ;

George, D ;

Eletr, S ;

Albrecht, G ;

Vermaas, E ;

Williams, SR ;

Moon, K ;

Burcham, T ;

Pallas, M ;

DuBridge, RB ;

Kirchner, J ;

Fearon, K ;

Mao, J ;

Corcoran, K .

NATURE BIOTECHNOLOGY, 2000, 18 (06) :630-634

[4]

BRENNER S, 2003, PLANT AN GEN 11 C SA

[5] Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome [J].

Brown, V ;

Jin, P ;

Ceman, S ;

Darnell, JC ;

O'Donnell, WT ;

Tenenbaum, SA ;

Jin, XK ;

Feng, Y ;

Wilkinson, KD ;

Keene, JD ;

Darnell, RB ;

Warren, ST .

CELL, 2001, 107 (04) :477-487

[6] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[7] Global and hormone-induced gene expression changes during shoot development in Arabidopsis [J].

Che, P ;

Gingerich, DJ ;

Lall, S ;

Howell, SH .

PLANT CELL, 2002, 14 (11) :2771-2785

[8]

CHO Y, 2002, GENOME BIOL, V3

[9] A systematic statistical linear modeling approach to oligonucleotide array experiments [J].

Chu, TM ;

Weir, B ;

Wolfinger, R .

MATHEMATICAL BIOSCIENCES, 2002, 176 (01) :35-51

[10]

Deyholos MK, 2001, CYTOMETRY, V43, P229, DOI 10.1002/1097-0320(20010401)43:4<229::AID-CYTO1055>3.3.CO

← 1 2 3 4 5 →