Purification of HTOS libraries by supercritical fluid chromatography

被引：13

作者：

Hochlowski, J ^{[1
]}

Olson, J ^{[1
]}

Pan, J ^{[1
]}

Sauer, D ^{[1
]}

Searle, P ^{[1
]}

Sowin, T ^{[1
]}

机构：

[1] Abbott Labs, Med Chem Technol, Abbott Pk, IL 60064 USA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2003年 / 26卷 / 03期

关键词：

supercritical fluid chromatography (SFC); high-throughput organic synthesis (HTOS); preparative chromatography;

D O I：

10.1081/JLC-120017174

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A commercially available preparative scale supercritical fluid chromatography (SFC) system has been customized and applied to the purification of high throughput organic synthesis (HTOS) libraries. Pilot reactions allow the triage between HPLC and SFC as the most appropriate purification technique, and when applicable, SFC has been proven to offer advantages in terms of decreased purification and sample dry-down time and purification capabilities, somewhat complementary to HPLC. Standardized chemistry-specific SFC methods have been determined and HTOS pilot reactions allow refinement of these standard methods to optimal conditions for a specific library core.

引用

页码：333 / 354

页数：22

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