Cell surface display of CD8 ecto domain on Escherichia coli using ice nucleation protein

被引：13

作者：

Kim, EJ

Yoo, SK

机构：

[1] Yonsei Univ, Coll Engn, Dept Biotechnol, Seoul 120749, South Korea

[2] Yonsei Univ, Bioprod Res Ctr, Seoul 120749, South Korea

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 03期

关键词：

D O I：

10.1023/A:1008865223017

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new system for cell surface display of recombinant proteins on Escherichia coli was tested for expression of the ecto domain of CD8, which is the surface protein of human T cytotoxic lymphocytes. Immunofluorescence microscopy, ELISA, and immunodot blotting confirmed successful expression of the CD8 ecto domain fused to ice nucleation protein (INP), an outer membrane protein of Pseudomonas syringae.

引用

收藏

页码：197 / 201

页数：5

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