Vaccination with Necroptotic Cancer Cells Induces Efficient Anti-tumor Immunity

被引：409

作者：

Aaes, Tania Love ^{[1
,2
]}

Kaczmarek, Agnieszka ^{[1
,2
]}

Delvaeye, Tinneke ^{[1
,2
,3
]}

De Craene, Bram ^{[2
,4
]}

De Koker, Stefaan ^{[2
,5
]}

Heyndrickx, Liesbeth ^{[1
,2
]}

Delrue, Iris ^{[1
,2
]}

Taminau, Joachim ^{[2
,4
]}

Wiernicki, Bartosz ^{[1
,2
]}

De Groote, Philippe ^{[1
,2
]}

Garg, Abhishek D. ^{[6
]}

Leybaert, Luc ^{[3
]}

Grooten, Johan ^{[2
,5
]}

Bertrand, Mathieu J. M. ^{[1
,2
]}

Agostinis, Patrizia ^{[6
]}

Berx, Geert ^{[2
,4
]}

Declercq, Wim ^{[1
,2
]}

Vandenabeele, Peter ^{[1
,2
,7
]}

Krysko, Dmitri V. ^{[1
,2
]}

机构：

[1] VIB, Mol Signaling & Cell Death Unit, Inflammat Res Ctr, B-9052 Ghent, Belgium

[2] Univ Ghent, Dept Biomed Mol Biol, CRIG, B-9052 Ghent, Belgium

[3] Univ Ghent, Dept Basic Med Sci, Physiol Grp, B-9000 Ghent, Belgium

[4] VIB, Inflammat Res Ctr, Mol & Cellular Oncol Unit, B-9052 Ghent, Belgium

[5] Univ Ghent, Dept Pharmaceut, B-9000 Ghent, Belgium

[6] KU Leuven Univ Leuven, Dept Cellular & Mol Med, Cell Death Res & Therapy Lab, B-3000 Leuven, Belgium

[7] Univ Ghent, Methusalem Program, B-9000 Ghent, Belgium

来源：

CELL REPORTS | 2016年 / 15卷 / 02期

关键词：

MIXED LINEAGE KINASE; DOMAIN-LIKE PROTEIN; CALRETICULIN EXPOSURE; ANTICANCER CHEMOTHERAPY; PROGRAMMED NECROSIS; MOLECULAR-PATTERNS; DEATH; APOPTOSIS; RIP3; IMMUNOGENICITY;

D O I：

10.1016/j.celrep.2016.03.037

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Successful immunogenic apoptosis in experimental cancer therapy depends on the induction of strong host anti-tumor responses. Given that tumors are often resistant to apoptosis, it is important to identify alternative molecular mechanisms that elicit immunogenic cell death. We have developed a genetic model in which direct dimerization of FADD combined with inducible expression of RIPK3 promotes necroptosis. We report that necroptotic cancer cells release damage-associated molecular patterns and promote maturation of dendritic cells, the cross-priming of cytotoxic T cells, and the production of IFN-gamma in response to tumor antigen stimulation. Using both FADD-dependent and FADD-independent RIPK3 induction systems, we demonstrate the efficient vaccination potential of immunogenic necroptotic cells. Our study broadens the current concept of immunogenic cell death and opens doors for the development of new strategies in cancer therapy.

引用

页码：274 / 287

页数：14

共 45 条

[1]

Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy [J].

Apetoh, Lionel ;

Ghiringhelli, Francois ;

Tesniere, Antoine ;

Obeid, Michel ;

Ortiz, Carla ;

Criollo, Alfredo ;

Mignot, Gregoire ;

Maiuri, M. Chiara ;

Ullrich, Evelyn ;

Saulnier, Patrick ;

Yang, Huan ;

Amigorena, Sebastian ;

Ryffel, Bernard ;

Barrat, Franck J. ;

Saftig, Paul ;

Levi, Francis ;

Lidereau, Rosette ;

Nogues, Catherine ;

Mira, Jean-Paul ;

Chompret, Agnes ;

Joulin, Virginie ;

Clavel-Chapelon, Francoise ;

Bourhis, Jean ;

Andre, Fabrice ;

Delaloge, Suzette ;

Tursz, Thomas ;

Kroemer, Guido ;

Zitvogel, Laurence .

NATURE MEDICINE, 2007, 13 (09) :1050-1059

[2]

Caspase-dependent immunogenicity of doxorubicin-induced tumor cell death [J].

Casares, N ;

Pequignot, MO ;

Tesniere, A ;

Ghiringhelli, F ;

Roux, S ;

Chaput, N ;

Schmitt, E ;

Hamai, A ;

Hervas-Stubbs, S ;

Obeid, M ;

Coutant, F ;

Métivier, D ;

Pichard, E ;

Aucouturier, P ;

Pierron, G ;

Garrido, C ;

Zitvogel, L ;

Kroemer, G .

JOURNAL OF EXPERIMENTAL MEDICINE, 2005, 202 (12) :1691-1701

[3]

Phosphorylation-Driven Assembly of the RIP1-RIP3 Complex Regulates Programmed Necrosis and Virus-Induced Inflammation [J].

Cho, YoungSik ;

Challa, Sreerupa ;

Moquin, David ;

Genga, Ryan ;

Ray, Tathagat Dutta ;

Guildford, Melissa ;

Chan, Francis Ka-Ming .

CELL, 2009, 137 (06) :1112-1123

[4]

Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury [J].

Degterev A. ;

Huang Z. ;

Boyce M. ;

Li Y. ;

Jagtap P. ;

Mizushima N. ;

Cuny G.D. ;

Mitchison T.J. ;

Moskowitz M.A. ;

Yuan J. .

Nature Chemical Biology, 2005, 1 (2) :112-119

[5]

Death receptor-induced apoptotic and necrotic cell death: differential role of caspases and mitochondria [J].

Denecker, G ;

Vercammen, D ;

Steemans, M ;

Vanden Berghe, T ;

Brouckaert, G ;

Van Loo, G ;

Zhivotovsky, B ;

Fiers, W ;

Grooten, J ;

Declercq, W ;

Vandenabeele, P .

CELL DEATH AND DIFFERENTIATION, 2001, 8 (08) :829-840

[6]

RIPK3 contributes to TNFR1-mediated RIPK1 kinase-dependent apoptosis in conditions of cIAP1/2 depletion or TAK1 kinase inhibition [J].

Dondelinger, Y. ;

Aguileta, M. A. ;

Goossens, V. ;

Dubuisson, C. ;

Grootjans, S. ;

Dejardin, E. ;

Vandenabeele, P. ;

Bertrand, M. J. M. .

CELL DEATH AND DIFFERENTIATION, 2013, 20 (10) :1381-1392

[7]

c-IAP1 and UbcH5 promote K11-linked polyubiquitination of RIP1 in TNF signalling [J].

Dynek, Jasmin N. ;

Goncharov, Tatiana ;

Dueber, Erin C. ;

Fedorova, Anna V. ;

Izrael-Tomasevic, Anita ;

Phu, Lilian ;

Helgason, Elizabeth ;

Fairbrother, Wayne J. ;

Deshayes, Kurt ;

Kirkpatrick, Donald S. ;

Vucic, Domagoj .

EMBO JOURNAL, 2010, 29 (24) :4198-4209

[8]

Therapeutic exploitation of necroptosis for cancer therapy [J].

Fulda, Simone .

SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 2014, 35 :51-56

[9]

A novel pathway combining calreticulin exposure and ATP secretion in immunogenic cancer cell death [J].

Garg, Abhishek D. ;

Krysko, Dmitri V. ;

Verfaillie, Tom ;

Kaczmarek, Agnieszka ;

Ferreira, Gabriela B. ;

Marysael, Thierry ;

Rubio, Noemi ;

Firczuk, Malgorzata ;

Mathieu, Chantal ;

Roebroek, Anton J. M. ;

Annaert, Wim ;

Golab, Jakub ;

de Witte, Peter ;

Vandenabeele, Peter ;

Agostinis, Patrizia .

EMBO JOURNAL, 2012, 31 (05) :1062-1079

[10]

Hypericin-based photodynamic therapy induces surface exposure of damage-associated molecular patterns like HSP70 and calreticulin [J].

Garg, Abhishek D. ;

Krysko, Dmitri V. ;

Vandenabeele, Peter ;

Agostinis, Patrizia .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 2012, 61 (02) :215-221

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