Activation of volume-regulated chloride currents by reduction of intracellular ionic strength in bovine endothelial cells

被引:109
作者
Nilius, B [1 ]
Prenen, J [1 ]
Voets, T [1 ]
Eggermont, J [1 ]
Droogmans, G [1 ]
机构
[1] Catholic Univ Louvain, Fysiol Lab, B-3000 Louvain, Belgium
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1998年 / 506卷 / 02期
关键词
D O I
10.1111/j.1469-7793.1998.353bw.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. We have studied the effects of intracellular ionic strength (Gamma(i)) on the swelling-activated whole-cell Cl- current (I-Cl,I-swell) in cultured calf pulmonary artery endothelial cells (CPAE cells). 2. Reducing Gamma(1) from 155 to 95 mM at constant osmolarity and Cl- concentration activates an outwardly rectifying current that is mainly carried by Cl- ions and inactivates at positive potentials. The amplitude of the current is larger at more reduced levels of Gamma(i). 3. The permeability ratio for the anions I-, Br-, Cl- and gluconate (P-I: P-Br: P-Cl: P-gluc) was 1.35:1.03:1:0.17. 4. Blockers of the swelling-activated Cl- current in CPAE cells also inhibit the current which is activated by a reduction in Gamma(i) with an IC50 of 1.1 mu M for tamoxifen, 1.3 mu M for mibefradil, and 35 mu M for quinidine. 5. The protein tyrosine kinase inhibitors tyrphostin B46 (50 mu M) and genistein (100 mu M), which inhibit I-Cl,I-swell in CPAE cells, also inhibited the Gamma(1)-induced current by 92.9 +/- 2.4% (n = 3) and 41.2 +/- 5.0% (n = 4), respectively. 6. Hypertonic extracellular solutions rapidly and reversibly antagonized the Gamma(i)-activated current, whereas increasing Gamma(i) from 155 to 195 mM precluded activation of I-Cl,I-swell by hypotonic shock. 7. It is concluded that a reduction of Gamma(i) activates an anion current that is identical to that activated by cell swelling. Changes in intracellular ionic strength may shift the volume set point for activation of I-Cl,I-swell.
引用
收藏
页码:353 / 361
页数:9
相关论文
共 21 条
  • [1] EMNMA F, 1997, AM J PHYSIOL, V272, pC1766
  • [2] Simultaneous measurement of membrane capacitance and whole cell currents during cell swelling in macrovascular endothelium
    Heinke, S
    Raskin, G
    DeSmet, P
    Droogmans, G
    VanDriessche, W
    Nilius, B
    [J]. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 1997, 7 (01) : 19 - 24
  • [3] Hoffmann EK, 1995, INT REV CYTOL, V161, P173
  • [4] Swelling-activated anion conductance in skate hepatocytes: Regulation by cell C1(-) and ATP
    Jackson, PS
    Churchwell, K
    Ballatori, N
    Boyer, JL
    Strange, K
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1996, 270 (01): : C57 - C66
  • [5] RED-CELL VOLUME REGULATION - THE PIVOTAL ROLE OF IONIC-STRENGTH IN CONTROLLING SWELLING-DEPENDENT TRANSPORT-SYSTEMS
    MOTAIS, R
    GUIZOUARN, H
    GARCIAROMEU, F
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1075 (02) : 169 - 180
  • [6] Inhibition by mibefradil, a novel calcium channel antagonist, of Ca2+- and volume-activated Cl- channels in macrovascular endothelial cells
    Nilius, B
    Prenen, J
    Kamouchi, M
    Viana, F
    Voets, T
    Droogmans, G
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1997, 121 (03) : 547 - 555
  • [7] ACTIVATION OF A CL- CURRENT BY HYPOTONIC VOLUME INCREASE IN HUMAN ENDOTHELIAL-CELLS
    NILIUS, B
    OIKE, M
    ZAHRADNIK, I
    DROOGMANS, G
    [J]. JOURNAL OF GENERAL PHYSIOLOGY, 1994, 103 (05) : 787 - 805
  • [8] Volume-activated Cl- channels
    Nilius, B
    Eggermont, J
    Voets, T
    Droogmans, G
    [J]. GENERAL PHARMACOLOGY-THE VASCULAR SYSTEM, 1996, 27 (07): : 1131 - 1140
  • [9] Multiple types of chloride channels in bovine pulmonary artery endothelial cells
    Nilius, B
    Sziics, G
    Heinke, S
    Voets, T
    Droogmans, G
    [J]. JOURNAL OF VASCULAR RESEARCH, 1997, 34 (03) : 220 - 228
  • [10] PERMEATION PROPERTIES AND MODULATION OF VOLUME-ACTIVATED CL- CURRENTS IN HUMAN ENDOTHELIAL-CELLS
    NILIUS, B
    SEHRER, J
    DROOGMANS, G
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 1994, 112 (04) : 1049 - 1056