Role of the outer membrane in the accumulation of quinolones by Serratia marcescens

被引：35

作者：

Berlanga, M

Ruiz, N

Hernandez-Borrell, J

Montero, T

Viñas, M

机构：

[1] Univ Barcelona, Div Hlth Sci, Microbiol Lab, Barcelona 08907, Spain

[2] Univ Barcelona, Sch Pharm, Lab Phys Chem 5, E-08028 Barcelona, Spain

来源：

CANADIAN JOURNAL OF MICROBIOLOGY | 2000年 / 46卷 / 08期

关键词：

D O I：

10.1139/cjm-46-8-716

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Accumulation of four quinolones by Serratia marcescens was measured fluorometrically. The passage of quinolones through the outer membrane was studied in both lipopolysaccharide-deficient and porin-deficient mutants. The lipopolysaccharide (LPS) layer formed a partially effective barrier for highly hydrophobic quinolones such as nalidixic acid. Quinolones with a low relative hydrophobicity coefficient seemed to pass preferentially through the water-filled Omp3 porin channels. Results were confirmed when Omp3 was cloned in a porin-defective Escherichia coli.

引用

页码：716 / 722

页数：7

共 46 条

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[2]

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