Development of a liquid chromatography-tandem mass spectrometry method for the identification of natural androgen steroids and their conjugates in urine samples

被引：50

作者：

Buiarelli, F

Coccioli, F

Merolle, M

Neri, B

Terracciano, A

机构：

[1] Univ Roma La Sapienza, Dipartimento Chim, I-00185 Rome, Italy

[2] Ist Zooprofilatt Sperimentale Reg Lazio & Toscana, I-00178 Rome, Italy

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 526卷 / 02期

关键词：

natural androgen steroids; conjugated hormones; LC-MS-MS; urine;

D O I：

10.1016/j.aca.2004.09.068

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An analytical procedure for the determination of natural steroids and their conjugates was developed and applied to bovine and human urine. Fifteen compounds (free and conjugated) after extraction by solid-phase were identified by liquid chromatography-tandem mass spectrometry (LC-MS-MS) in multiple reaction monitoring (MRM). Free and conjugated steroids were detected all together in the same chromatographic analysis respectively in positive and negative ionization. This procedure reveals to be time-saving, because it overcomes the need of further sample pre-treatment steps, such as enzymatic hydrolysis and derivatisation. The recovery for each compound was around 80%. Limit of detection of each steroid free or conjugated has been evaluated. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：113 / 120

页数：8

共 15 条

[1] Testing for natural and synthetic anabolic agents in human urine [J].