Stimulation of glutamate receptor protein synthesis and membrane insertion within isolated neuronal dendrites

被引：103

作者：

Kacharmina, JE

Job, C

Crino, P

Eberwine, J ^{[1
]}

机构：

[1] Univ Penn, Med Ctr, Dept Pharmacol & Psychiat, Philadelphia, PA 19104 USA

[2] Univ Penn, Med Ctr, Dept Neurol, Philadelphia, PA 19104 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 21期

关键词：

D O I：

10.1073/pnas.97.21.11545

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The selective subcellular localization of mRNAs to dendrites and the recent demonstration of local protein synthesis have highlighted the potential role of postsynaptic sites in modulation of cell-cell communication. We show that epitope-tagged subunit 2 of the ionotopic glutamate receptor, GluR2, mRNA transfected into isolated hippocampal neuronal dendrites is translated in response to pharmacologic stimulation. Further, confocal imaging of N-terminally labeled GluR2 reveals that the newly synthesized GluR2 protein can integrate into the dendritic membrane with the N terminus externally localized, These data demonstrate that integral membrane proteins can be synthesized in dendrites and can locally integrate into the cell membrane.

引用

页码：11545 / 11550

页数：6

共 28 条

[1] Metabotropic glutamate receptor-initiated translocation of protein kinase p90rsk to polyribosomes:: A possible factor regulating synaptic protein synthesis [J].