A microfluidic system for saliva-based detection of infectious diseases

被引：65

作者：

Chen, Zongyuan

Mauk, Michael G.

Wang, Jing

Abrams, William R.

Corstjens, Paul L. A. M.

Niedbala, R. Sam

Malamud, Daniel

Bau, Haim H.

机构：

[1] Univ Penn, Sch Engn & Appl Sci, Philadelphia, PA 19104 USA

[2] NYU, Coll Dent, New York, NY USA

[3] Leiden Univ, Med Ctr, Leiden, Netherlands

[4] Lehigh Univ, Dept Chem, Bethlehem, PA 18015 USA

来源：

ORAL-BASED DIAGNOSTICS | 2007年 / 1098卷

关键词：

microfluidics; lab-on-a-chip; infectious diseases; diagnostics; immunochromatography;

D O I：

10.1196/annals.1384.024

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

A '' lab-on-a-chip '' system for detecting bacterial pathogens in oral fluid samples is described. The system comprises: (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette ('' chip '') for sample processing including immunochromatographic assay with a nitrocellulose lateral now strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product With bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.

引用

页码：429 / 436

页数：8

共 10 条

[1]

CHEN Z, 2005, P 9 INT C MIN SYS CH, P791

[2]

Corstjens P, 2001, CLIN CHEM, V47, P1885

[3]

Corstjens P. L. A. M., 2005, IEE Proceedings Nanobiotechnology, V152, P64, DOI 10.1049/ip-nbt:20045014

[4] Lateral-flow and up-converting phosphor reporters to detect single-stranded nucleic acids in a sandwich-hybridization assay [J].