Differential expression and cellular localization of somatolactin-1 and-2 during early development in the gilthead sea bream

The patterns of expression of the somatolactin 1 and 2 (SL1 and SL2) transcripts were studied during the early development of the gilthead sea bream (Sparus aurata). Gene expression of SL1 and SL2 were detected in embryos and in larvae, although both transcripts presented different levels of expression. The SLI transcripts in contrast to the SL2 transcripts presented high expression levels in embryos and younger larvae. Moreover, the SL2 transcripts were slightly present or absence in embryonic stage and the newly hatched larvae, respectively. The differences in the expression levels of SL1 and SL2 in embryos and larvae may be due to the fact that two distinct genes express both isoforms of the protein. Thus, both SLs may play different physiological roles throughout development. Moreover, the hybridization signals for SL1- and SL2-mRNAs were detected in 4-day-old larvae. Both in larvae and adults the somatolactotroph cells co-expressed both transcripts of SL and were located bordering the neurohypophysis in the pars intermedia. (C) 2003 Elsevier Science (USA). All rights reserved.