Members of the heterogeneous nuclear ribonucleoprotein H family activate splicing of an HIV-1 splicing substrate by promoting formation of ATP-dependent spliceosomal complexes

被引：86

作者：

Schaub, Michael C. ^{[1
]}

Lopez, Suzette R. ^{[1
]}

Caputi, Massimo ^{[1
]}

机构：

[1] Florida Atlantic Univ, Dept Biomed Sci, Boca Raton, FL 33431 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2007年 / 282卷 / 18期

关键词：

D O I：

10.1074/jbc.M700774200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study we analyzed members of the heterogeneous nuclear ribonucleoprotein ( hnRNP) H protein family to determine their RNA binding specificities and roles in splicing regulation. Our data indicate that hnRNPs H, H', F, 2H9, and GRSF-1 bind the consensus motif DGGGD (where D is U, G, or A) and aggregate in a multimeric complex. We analyzed the role of these proteins in the splicing of a substrate derived from the HIV-1 tat gene and have shown that hnRNP H family members are required for efficient splicing of this substrate. The hnRNP H protein family members activated splicing of the viral substrate by promoting the formation of ATP-dependent spliceosomal complexes. Mutational analysis of six consensus motifs present within the intron of the substrate indicated that only one of these motifs acts as an intronic splicing enhancer.

引用

页码：13617 / 13626

页数：10

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