Detection of antitumor promoting activity in Raji cells carrying Epstein-Barr virus genome by immunoblotting analysis

被引：9

作者：

Kondo, A ^{[1
]}

Morimoto, T ^{[1
]}

Okazaki, K ^{[1
]}

机构：

[1] Kagawa Univ, Fac Agr, Dept Bioresource Sci, Kagawa 7610795, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 02期

关键词：

antitumor promoter; Epstein-Barr virus early antigen; Raji cells; P3HR-1; cells; TPA;

D O I：

10.1271/bbb.62.377

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Extract of Raji cells treated with sodium n-butyrate (1 mM) and a tumor promoter, 12-O-tetradecanoylphorbol-13-acetate (TPA, 40 ng/ml), was analyzed by immunoblotting using ten human sera with different antibody titers against Epstein-Barr virus early antigens. Two human sera reacted with one induced polypeptide of 48 kDa and its induction was inhibited by curcumin (4 mu g/ml), an antitumor promoter from turmeric. A mouse antiserum against P3HR-1. cells treated with TPA and sodium n-butyrate also detected the 48-kDa polypeptide in Raji cells treated with TPA at concentrations of 2.5 to 80 ng/ml. These results indicate that the immunoblotting analysis can be used in a confirmation test for detection of antitumor promoting activity.

引用

页码：377 / 379

页数：3

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